上感颗粒调节肺炎链球菌感染性肺炎小鼠炎症反应的研究  

Study of Shanggan Granules on Regulating Inflammatory Action in Mice with Streptococcus Pneumoniae Pneumonia

作　　者：李津金[1] 王毅军[1] 芮庆林[1] 王海丹[1] 奚肇庆[1] Li Jinjin;Wang yijun;Rui Qinglin;Wang Haidan;Xi Zhaoqing(Jiangsu Province Hospital of Chinese Medicine,Jiangsu,Nanjing 210029,China)

机构地区：[1]江苏省中医院,江苏南京210029

出　　处：《中国中医急症》2021年第11期1932-1935,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：江苏省名老中医药专家奚肇庆传承工作室建设项目(苏中医教科[2016]6号);江苏省中医院科技项目(Y14057)。

摘　　要：目的观察上感颗粒对肺炎链球菌感染性肺炎小鼠外周血CD4+、CD8+T淋巴细胞、CD4+/CD8+,血清白细胞介素-2(IL-2)、可溶性白细胞介素-2受体(SIL-2R)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)的影响,探讨上感颗粒治疗肺炎链球菌肺炎的免疫调节机制。方法肺炎链球菌感染性肺炎小鼠随机分成6组,测定给药第3天末、第6天末小鼠CD4+、CD8+T细胞、CD4+/CD8+水平,观察给药第3天末、第6天末小鼠IL-2、SIL-2R、IL-6、TNF-α水平的变化。结果上感颗粒的小剂量组、大剂量组、大剂量联合左氧氟沙星组均能降低肺炎链球菌感染性肺炎小鼠外周血CD4+、CD8+T细胞、血清SIL-2R、IL-6、TNF-α水平,升高IL-2水平,与模型组比较差异具有统计学意义(P<0.05)。结论上感颗粒可减少肺炎链球菌肺炎小鼠外周血CD4+、CD8+T细胞,降低血清SIL-2R、IL-6、TNF-α水平,升高血清IL-2水平,提示其具有免疫调节作用。Objective:To observe the effects of Shanggan Granules on peripheral blood CD4+,CD8+T lymphocytes,CD4+/CD8+,serum interleukin-2(IL-2),and soluble interleukin-2(SIL-2 R),IL-6,and tumor necrosis factor-α(TNF-α)in mice with pneumonia caused by streptococcus pneumoniae,and to explore the immunoregulatory mechanism of Shanggan Granules in the treatment of pneumonia caused by streptococcus pneumoniae.Methods:Mice with streptococcus pneumoniae pneumonia were randomly divided into 6 groups,and the levels of CD4+,CD8+T cells,CD4+/CD8+at the end of the 3 rd day of administration were measured,and the changes of IL-2,SIL-2 R,IL-6,and TNF-αof the mice were observed at the end of the 3 rd day and at the end the 6 th day after administration.Results:The low-dose,high-dose,and high-dose combined with levofloxacin group of Shanggan Granules could reduce peripheral blood CD4+,CD8+T cells,serum SIL-2 R,IL-6,and TNF-αin mice with pneumonia caused by streptococcus pneumoniae.The level of IL-2 increased,and the difference was statistically significant compared with the model group(P<0.05).Conclusion:Shanggan Granules can reduce peripheral blood CD4+and CD8+T cells in mice with streptococcus pneumoniae pneumonia,reduce serum SIL-2 R,IL-6,TNF-αlevels,and increase serum IL-2 levels,suggesting that it has an immunomodulatory effect.

关 键 词：肺炎链球菌感染性肺炎 上感颗粒 CD4+ CD8+ 白细胞介素-2 可溶性白细胞介素-2受体 白细胞介素-6 肿瘤坏死因子-α 小鼠 

分 类 号：R285.5[医药卫生—中药学]