依达拉奉对缺氧缺血性脑病大鼠脑组织MMP-9、MMP-3、SOD、MDA表达的影响  

Effects of Edaravone on the Expression of MMP-9,MMP-3,SOD and MDA in Brain Tissue of Rats with Hypoxic-Ischemic Brain Damage

作　　者：张晶[1] 谷丽彩 李燕燕 赵晓彦[1] 董燕[1] 张君[2] ZHANG Jing;GU Li-cai;LI Yan-yan;ZHAO Xiao-yan;DONG Yan;ZHANG Jun(Department of Pediatrics,the First Hospital of Hebei Medical University,Shijiazhuang 050031,China;Cell Therapy Laboratory,First Hospital of Hebei Medical University,Shijiazhuang 050031,China)

机构地区：[1]河北医科大学第一医院儿科,河北石家庄050031 [2]河北医科大学第一医院细胞治疗实验室,河北石家庄050031

出　　处：《药物生物技术》2021年第4期381-384,共4页Pharmaceutical Biotechnology

基　　金：河北省科技厅河北省重点研发计划项目健康医疗与生物医药专项(No.182777109D);河北医科大学第一医院“星火”青年科研项目(No.XH201712)。

摘　　要：探讨依达拉奉对缺氧缺血性脑病大鼠脑(H1BD)组织MMP-9、MMP-3、SOD、MDA表达的影响。选择90只清洁级新生雄性Wistar大鼠,按随机数表法分成假手术组30只及HIBD组30只、依达拉奉治疗组30只。经Western Blot法测定各组大鼠脑组织MMP-9及MMP-3蛋白表达,经黄嘌呤氧化镁法测定大鼠脑组织SOD活性,并通过TBA比色法检测大鼠脑组织MDA含量。HIBD组大鼠缺血缺氧6,24,48,72,120,168 h时脑组织含水量高于假手术组、依达拉奉治疗组(P<0.05);HIBD组大鼠缺血缺氧6,24,48,72,120,168 h时脑组织MMP-9、MMP-3蛋白相对丰度值高于假手术组、依达拉奉治疗组(P<0.05);HIBD组大鼠缺血缺氧6,24,48,72,120,168 h时脑组织SOD活性低于假手术组、依达拉奉治疗组(P<0.05);HIBD组大鼠缺血缺氧6,24,48,72,120,168 h时脑组织MDA表达高于假手术组、依达拉奉治疗组(P<0.05)。MMP-9、MMP-3、SOD、MDA参与缺氧缺血性脑病发生及进展,应用依达拉奉治疗能减少MMP-9、MMP-3及MDA表达,增强SOD活性,进而缓解脑水肿,分析可能是依达拉奉与保护脑组织作用机制有关。To investigate the effects of edaravone on the expression of MMP-9,MMP-3,SOD and MDA in the brain of rats with Hypoxic-ischemic brain damage,90 cases of clean male wistar rats were randomly divided into 30 sham operation group,30 HIBD group and 30 edaravone treatment group.The expressions of MMP-9 and MMP-3 in brain tissue of rats in each group were determined by Western Blot.The activity of SOD in brain tissue was determined by Huangqi magnesium oxide method.The content of MDA in rat brain tissue was detected by TBA colorimetry.The water content of brain tissue in the HIBD group was higher than that in the sham operation group and edaravone treatment group at 6,24,48,72,120,168 h(P<0.05).The relative abundance of MMP-9 and MMP-3 protein in the HIBD group at 6,24,48,72,120,168 h was higher than that in the sham operation group and edaravone treatment group(P<0.05).The activities of SOD in brain tissue of HIBD group were lower than that of sham operation group and edaravone treatment group at 6,24,48,72,120,168 h(P<0.05).The expression of MDA in brain tissue of HIBD group was higher than that of sham operation group and edaravone treatment group at 6,24,48,72,120,168 h(P<0.05).MMP-9,MMP-3,SOD and MDA were involved in the development and progression of Hypoxic-ischemic brain damage.Treatment with edaravone could reduce the expression of MMP-9,MMP-3 and MDA,enhanced SOD activity,and relieved brain edema.The analysis might be the mechanism by which edaravone protected brain tissue.

关 键 词：依达拉奉 缺氧缺血性脑病 大鼠 基质金属蛋白酶-9 基质金属蛋白酶-3 超氧化物歧化酶 丙二醛 

分 类 号：R965[医药卫生—药理学]