基质金属蛋白酶-1基因多态性与脊柱结核易感性及手术预后的关联性  

Association of matrix metalloproteinase-1gene polymorphism with susceptibility to spinal tuberculosis and prognosis of surgery

作　　者：周凤珍[1] 葛梅[1] 李伟[2] 汤样华[3] 陈敏丽[1] ZHOU Feng-zhen;GE Mei;LI Wei;TANG Yang-hua;CHEN Min-li(Xiaoshan District Hospital of Traditional Chinese Medicine,Hangzhou City,Zhejiang Province,Hangzhou,Zhejiang310000,China)

机构地区：[1]浙江省杭州市萧山区中医院手术室,浙江杭州310000 [2]浙江省杭州市萧山区中医院脊柱外科,浙江杭州310000 [3]浙江省杭州市萧山区中医院骨科,浙江杭州310000

出　　处：《中华医院感染学杂志》2021年第19期2959-2963,共5页Chinese Journal of Nosocomiology

基　　金：浙江省医药卫生科技基金资助项目(2019KY548)。

摘　　要：目的探讨基质金属蛋白酶-1(MMP-1)基因多态性与脊柱结核易感性及手术预后的关联性。方法选择杭州市萧山区中医院2017年1月-2020年1月接受手术治疗的脊柱结核患者86例设为研究组,选择同期接受脊柱手术但未发生脊柱结核感染的患者90例设为对照组。抽取两组患者外周静脉血提取DNA后,采用聚合酶链式扩增反应(PCR)对模板中DNA序列进行扩增,扩增结果送公司进行测序。采用酶联免疫吸附法(ELISA)检测血清中MMP-1、C-反应蛋白(CRP)、血沉(ESR)水平。结果研究组MMP-1基因1607位点2G/2G基因型、2G等位基因频率(31.40%、48.26%)高于对照组(P<0.05);MMP-1-1607位点显性模型、隐性模型和加性模型比较,差异有统计学意义(P<0.05);携带1G/1G、1G/2G、2G/2G基因型研究组患者MMP-1、CRP、ESR水平差异有统计学意义(P<0.05),2G/2G组MMP-1、CRP、ESR水平分别为:(74.46±16.24)ng/ml、(17.16±4.44)mg/L、(37.82±6.42)mm/h均高于1G/2G组及1G/1G组,三组预后不良发生率差异无统计学意义(P=0.848)。结论MMP-1基因1607位点基因突变可能与本地区居民对脊柱结核易感性有关,2G等位基因为易感基因,但与手术预后无关。OBJECTIVE To explore the relationship of matrix metalloproteinase-1(matrix metalloproteinase-1,MMP-1)gene polymorphism,spinal tuberculosis susceptibility,and prognosis of surgery.METHODS The 86patients with spinal tuberculosis who underwent surgery from Jan.2017to Jan.2020in Xiaoshan District Hospital of Hangzhou City were selected as the study group,and 90patients who underwent spinal surgery but did not develop spinal tuberculosis infection were selected as the control group.After extracting DNA from peripheral venous blood of two groups of patients,polymerase chain amplification reaction(PCR)was used to amplify the DNA sequence in the template,and the amplification results were sent to the company for sequencing.Enzyme-linked immunosorbent assay(ELISA)was used to detect serum levels of MMP-1,C-reactive protein(CRP),and erythrocyte sedimentation rate(ESR).RESULTS The frequency of 2G/2Ggenotypes and 2Galleles(31.40%,48.26%)of MMP-1gene 1607in the study group were significantly higher than those in the control group(P<0.05).Comparison among MMP-1-1607locus dominant model,recessive model and the additive model showed significant differences(P<0.05);the MMP-1,CRP,and ESR levels of patients in the study group carrying 1G/1G,1G/2G,and 2G/2Ggenotypes were significant(P<0.05).The levels of MMP-1,CRP and ESR in the 2G/2Ggroup were(74.46±16.24)ng/ml,(17.16±4.44)mg/L,and(37.82±6.42)mm/h,respectively,all higher than those in the 1G/2Gand 1G/1Ggroup,there was no significant difference in the incidence of poor prognosis among the three groups(P=0.848).CONCLUSION MMP-1gene mutation at locus 1607might be related to the susceptibility of local residents to spinal tuberculosis.The 2Gallele was a susceptible gene,but it had nothing to do with the prognosis of surgery.

关 键 词：基质金属蛋白酶 基因多态性 脊柱结核 手术预后 发病风险 

分 类 号：R681.5[医药卫生—骨科学]