基于D-甘露糖和葡萄糖含量的白木通种质资源评价  

Evaluation of Akebia trifoliata(Thunb.) Koidz. var. australis(Diels) Rehd.Germplasm Resources Based on D-mannose and Glucose Content

作　　者：李园园 陈松树 黄明进[1,3] 刘红昌 罗培高[4] 郑重 Li Yuanyuan;Chen Songshu;Huang Mingjin;Liu Hongchang;Luo Peigao;Zheng Zhong(College of Agriculture,Guizhou University,Guiyang,550025;Teaching Experiment Farm,Guizhou University,Guiyang,550025;Key Laboratory of Guizhou for Propagation and Cultivation of Medicinal Plants,Guiyang,550025;College of Agronomy,Sichuan Agricultural University,Wenjiang,611130;Tian Di Zai Ecological Agriculture Co.,Ltd in Chengdu City,Chengdu,610000)

机构地区：[1]贵州大学农学院,贵阳550025 [2]贵州大学教学实验场,贵阳550025 [3]贵州省药用植物繁育与种植重点实验室,贵阳550025 [4]四川农业大学农学院,温江611130 [5]成都市天地载生态农业有限公司,成都610000

出　　处：《基因组学与应用生物学》2021年第4期1771-1777,共7页Genomics and Applied Biology

基　　金：贵州省自然科学基金(黔科合基础[2019]1061号);贵州省生物学一流学科建设项目(GNYL[2017]009)共同资助。

摘　　要：本研究以D-甘露糖和葡萄糖含量作为定点评价指标对白木通种Akebia trifoliata (Thunb.) Koidz. var.australis (Diels) Rehd.质资源进行评价,筛选葡萄糖含量/D-甘露糖含量(C葡/C甘)比值较低且稳定的白木通种质。采用RP-HPLC系统检测白木通不同种质果皮中的D-甘露糖和葡萄糖含量。研究结果表明,在两年的同一区域试验点和不同区域试验点上白木通种质‘黔通1号’的D-甘露糖含量均表现最高,C葡/C甘比值均较低,但各个区域试验点的结果差异较大,达到显著及极显著水平;白木通种质‘黔通2号’和‘黔通3号’的D-甘露糖和葡萄糖含量在同一试验点和不同试验点上呈不稳定状态。三份白木通种质D-甘露糖和葡萄糖含量受环境因子的影响大于其自身遗传因子的影响‘,黔通1号’种质D-甘露糖和葡萄糖代谢遗传稳定性较‘黔通2号’和‘黔通3号’种质稳定,适合作药用种质加以推广。The aim of this study was to select Akebia trifoliata(Thunb.) Koidz. var. australis(Diels) Rehd.germplasm with lower and more stable ratio of glucose content/D-mannose content(CG/CD) by evaluating Akebia trifoliata(Thunb.) Koidz. var. australis(Diels) Rehd. germplasm resources based on the content of D-mannose and glucose. D-mannose and glucose content of peels in different A. trifoliata(Thunb.) Koidz. var. australis(Diels)Rehd. germplasms was measured by RP-HPLC system. The results showed that D-mannose content of A. trifoliata(Thunb.) Koidz. var. australis(Diels) Rehd. germplasm-’Qiantong No.1’ was the highest in the same regional test site and different regional test sites and with lower CG/CDratio in 2 years. However, the result difference was major and showed significant and highly significant difference in different regional test sites;D-mannose and glucose content of A. trifoliata(Thunb.) Koidz. var. australis(Diels) Rehd. germplasm-’Qiantong No.2’ and ’Qiantong No.3’ showed unstable state in the same regional test site and different regional test sites. The effect of environmental factors to the content of D-mannose and glucose of ’Qiantong No.1’,’Qiantong No.2’ and ’Qiantong No.3’ was greater than that of the genetic factors itself. The stability of D-mannose and glucose metabolism of ’Qiantong No.1’ was stabler than that of ’Qiantong No.2’, ’Qiantong No.3’, which was more suitable for planting as medicinal germplasm.

关 键 词：白木通 种质 甘露糖 葡萄糖 

分 类 号：S567.19[农业科学—中草药栽培]