金鱼草AmHMGS基因的克隆及表达特征分析  

Cloning and Characterization of Am HMGS Gene from Antirrhinum majus

作　　者：邵慧慧 王少杰[1] 韩嘉宁 胡增辉[1,2] 冷平生 Shao Huihui;Wang Shaojie;Han Jianing;Hu Zenghui;Leng Pingsheng(College of Landscape Architecture,Beijing Agricultural College,Beijing,102206;Beijing Advanced Innovation Center for Tree Breeding by Molecular Design,Beijing,102206)

机构地区：[1]北京农学院园林学院,北京102206 [2]北京林木分子设计育种高精尖创新中心,北京102206

出　　处：《基因组学与应用生物学》2021年第4期1778-1785,共8页Genomics and Applied Biology

基　　金：国家自然科学基金(31201645);北京市自然科学基金(6172006);北京市属高等学校创新团队建设与教师职业发展计划项目(IDHT20180509);北京市教委-自然科学基金委联合科技计划重点项目(KZ201800200)共同资助。

摘　　要：植物萜烯类化合物合成主要通过MVA和MEP途径,这些萜烯类化合物在植物生长、发育过程发挥着重要作用,萜烯类化合物在植物花香挥发成分中占有大比率。3-羟基-3-甲基戊二酰辅酶A合成酶基因(HMGS)是MVA途径中的关键酶基因,该酶作用主要是催化底物乙酰辅酶A和乙酰乙酰辅酶A生成3-羟基-3-甲基戊二酰辅酶A (HMG-CoA),是合成萜烯类化合物的前体限速酶。本研究以‘马里兰’金鱼草(Antirrhinum majus’Maryland’)为材料,克隆AmHMGS,基因全长1 383 bp,编码460个氨基酸,时空和组织特异性RT-qPCR表达分析表明,AmHMGS基因在花中表达量显著高于根茎叶;初开期的表达量最高;在盛花期的不同花器官中,Am HMGS基因在雄蕊和上瓣中表达量最高,与其它花器官中的表达量差异显著。用茉莉酸抑制剂不同浓度的菲尼酮处理盛开期金鱼草花瓣,RT-qPCR分析HMGS基因表达量结果表明,菲尼酮处理后能抑制该基因的表达。本研究的结果为明确AmHMGS基因在金鱼草中的表达模式,为今后研究该基因的功能及其在金鱼草花香释放中的信号作用奠定基础。The synthesis of plant terpenoids is mainly through the MVA and MEP pathways. These terpenoids play an important role in plant growth and development, and also contain a large amount of terpenoids in plant floral volatiles. 3-hydroxy-3-methylglutaryl coenzyme A synthetase gene(HMGS) is a key enzyme gene in the MVA pathway, which mainly catalyzes the condensation of acetyl-CoA and acetoacetyl-CoA into 3-hydroxy-3-methyl Lipoglutaryl Coenzyme A(HMG-CoA) is a precursor rate-limiting enzyme for the synthesis of terpenes. In this study, Am HMGS was cloned from Antirrhinum majus ’Maryland’. The gene was 1 383 bp in length and encoded 460 amino acids. The expression of AmHMGS gene in flowers was expressed by spatio-temporal and tissue-specific RT-qPCR expression analysis. The expression level of AmHMGS gene in flowers was significantly higher than that in roots and leaves;the expression level was the highest in the initial stage;in the different flower organs of the flowering stage, the AmHMGS gene had the highest expression in the stamen and upper lobes, and the expression level in other flower organs was significantly different. The jasmonic acid inhibitors treated with different concentrations of fenidone were used to treat the petals of Snapdragon, and the expression of HMGS gene by RT-qPCR showed that fenidone could inhibit the expression of genes. The results of this study were to clarify the expression pattern of Am HMGS gene in Snapdragon, and lay a foundation for further study of the function of this gene and its signalling role in the floral release of snapdragon.

关 键 词：‘马里兰’金鱼草 基因克隆 AmHMGS 表达分析 

分 类 号：S682.19[农业科学—观赏园艺]