过表达SmERF1提高了丹参耐盐性  

Overexpression of Sm ERF1 Improves Salt Tolerance in Salvia miltiorrhiza

作　　者：化文平 李世强[1] 孔维维 李翠芹[2] Hua Wenping;Li Shiqiang;Kong Weiwei;Li Cuiqin(Department of Life Sciences and Food Technology,Shaanxi Xueqian Normal University,Xi'an,710061;Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry,Ministry of Education,National Engineering Laboratoiy for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China,College of Life Sciences,Shaanxi Normal University,Xi'an,710062)

机构地区：[1]陕西学前师范学院生命科学与食品工程学院,西安710061 [2]陕西师范大学生命科学学院,西北濒危药材资源开发国家工程实验室,药用资源与天然药物化学教育部重点实验室,西安710062

出　　处：《基因组学与应用生物学》2021年第4期1786-1792,共7页Genomics and Applied Biology

基　　金：国家重点研发计划中医药现代化研究专项(2019YFC1712602);陕西省重点研发计划项目(2020SF-314);陕西省科技厅自然科学基金项目(2019JQ-917);陕西省大学生创新训练项目(S201914390022)共同资助。

摘　　要：ERF (ethylene-responsive factor)蛋白是植物中一大类转录因子家族,在植物的生长发育及逆境胁迫中发挥着重要作用。为研究丹参中新的转录因子SmERF1的功能,本研究对其编码序列、表达模式进行分析,并在丹参中进行过表达,检测过表达后转基因植株耐盐性、次生代谢物和激素的变化。研究结果表明,SmERF1含有一个AP2结构域,是典型的ERF转录因子。SmERF1受到MeJA和酵母诱导子(YE)的诱导表达。在盐胁迫下,过表达SmERF1的丹参株系中脯氨酸含量、SOD和POD活性较野生型丹参植株高,MDA含量降低,说明转基因株系的抗盐特性增强。而且过表达SmERF1的丹参株系中ABA含量升高,GA含量降低;过表达Sm ERF1对丹参酮、丹酚酸等代谢物的合成调控效果不强。综合以上结果,表明Sm ERF1可能通过调节ABA的合成,提高了丹参耐盐性。ERF(ethylene-responsive factor) proteins are a large family of transcription factors in plants, which play an important role in plant growth and development. In order to study the function of a novel transcription factor SmERF1 in Salvia miltiorr-hiza, the coding sequence and expression pattern of SmERF1 were analyzed. And the changes of resistance, seco-ndary metabolites and hormones of SmERF1-overexpression transgenic plants were also detected. The results showed that SmERF1 contains only one AP2 domain, which is a typical ERF transcription factor. The expression of SmERF1 was induced by Me JA and yeast elicitor(YE). The proline contents, SOD and POD activities of the Sm ERF1-overexpression lines were higher than those of wild-type plants, and the MDA contents decreased after NaCl treatment, which indicated that the salt resistance of the transgenic lines were enhanced. The plant hormone ABA contents were up-regulated and the GA contents decreased in SmERF1-overexpression transgenic plant. But the regulation effect by SmERF1 overexpression on the biosynthesis of tanshinones or salvianolic acids, was not strong. These results indicated that SmERF1 improved salt tolerance in S. miltiorrhiza by regulating ABA biosynthesis.

关 键 词：丹参 ERF ABA 耐盐性 次生代谢物 

分 类 号：S567.53[农业科学—中草药栽培]