HPLC法测定复方冻疮乳膏中维生素E及消旋山莨菪碱的含量  

Determination of vitamin E and raceanisodamine in Chilblain Cream by HPLC

作　　者：肖琪[1] 徐健峰 李步阳[1] XIAO Qi;XU Jian-feng;LI Bu-yang(Jiangsu Hospital of integrated traditional Chinese and Western Medicine,Nanjing 210028,China)

机构地区：[1]江苏省中西医结合医院,南京210028 [2]南京康舟医药科技有限公司,南京211100

出　　处：《中国处方药》2021年第11期21-23,共3页Journal of China Prescription Drug

摘　　要：目的建立以高效液相色谱法同时测定冻疮乳膏中维生素E及消旋山莨菪碱的含量。方法色谱柱为Agilent ZORBAX SB C_(18)(250 mm×4.6 mm,5μm);流动相A:0.01%三乙胺;流动相B:甲醇;梯度洗脱,维生素E检测波长为280 nm,消旋山莨菪碱检测波长为220 nm,流速为1.0 ml/min。结果消旋山莨菪碱和维生素E分别在80~120μg/ml和4~6 mg/ml浓度范围内与峰面积呈良好的线性关系(相关系数r均为0.9999);消旋山莨菪碱的平均回收率为99.33%,相对标准偏差为0.44%(n=9);维生素E的平均回收率为99.26%,相对标准偏差为0.67%(n=9)。消旋山莨菪碱的平均含量为99.44%,相对标准偏差为0.57%(n=12);维生素E的平均含量为99.16%,相对标准偏差为0.89%(n=12)。结论该方法专属、精密、准确、简便,可用于冻疮乳膏中维生素E及消旋山莨菪碱的含量测定。Objective To establish a HPLC method for simultaneous determination of vitamin E and raceanisodamine in frostbite cream.Methods The HPLC method was Agilent ZORBAX SB C_(18) column(250 mm×4.6 mm,5μm);Flow phase A:0.01%triethylamine;Flow phase B:methanol;Gradient elution,Vitamin E detection wavelength 280 nm,Detection wavelength of raceanisodamine 220 nm,Flow 1.0 ml/min.Results Raceanisodamine and vitamin E showed good linear relationship with peak area in the concentration range of 80μg/ml~120μg/ml and 4 mg/ml~6 mg/ml respectively(correlation coefficient r=0.9999);The average recovery of raceanisodamine was 99.33%,The relative standard deviation was 0.44%(n=9),Average recovery of vitamin E was 99.26%,a relative standard deviation of 0.67%(n=9).The mean content of raceanisodamine was 99.44%,a relative standard deviation of 0.57%(n=12),Average vitamin E is 99.16%,a relative standard deviation of 0.89%(n=12).Conclusion This method is specific、precise、accurate and simple,and can be used for the determination of vitamin E and raceanisodamine in frostbite cream.

关 键 词：HPLC法 复方冻疮乳膏 维生素E 消旋山莨菪碱 含量测定 

分 类 号：R927.2[医药卫生—药学]