RP-HPLC-UV法测定半夏泻心汤物质基准中黄芩苷的含量  

Determination of Baicalin in Banxia Xiexin Decoction by RP-HPLC-UV

作　　者：闫丽莉[1] 宋小雪 陈忠新[1] 张文娓[1] 田明[1] YAN Li-li;SONG Xiao-xue;CHEN Zhong-xin;ZHANG Wen-wei;TIAN Ming(Experimental Training Center,Heilongjiang University of Traditional Chinese Medicine,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《化学工程师》2021年第11期28-31,共4页Chemical Engineer

基　　金：黑龙江中医药大学“中药经典名方半夏泻心汤制剂研发”科研项目(22012180003);黑龙江中医药大学校科研基金资助项目“基于经典名方半夏泻心汤有效成分传递关系的研究”(2019MS38)。

摘　　要：目的采用高效液相色谱连用紫外检测器检测经典名方半夏泻心汤中黄芩苷含量。方法色谱柱以十八烷基硅烷键合硅胶为填充剂,以0.22%磷酸水为流动相A,甲醇为流动相B,比例为53∶47,流速设置为1mL·min^(-1),检测波长为280nm。结果黄芩苷出峰时间为10min左右,峰形良好且无其他物质干扰,黄芩苷含量在0.26~1.56μg之间呈良好线性关系,得回归方程为:Y=60.999X-1.3235,R^(2)=0.9998。最终确定半夏泻心汤物质基准的黄芩苷每剂含量范围309.23~574.28mg。结论该方法有效简便的测定出半夏泻心汤物质基准中黄芩苷的含量,为经典名方半夏泻心汤的进一步研究与发展奠定基础。OBJECTIVE In order to determine the content of Baicalin in Banxia Xiexin Decoction by HPLC with UV detector.METHODS The chromatographic column was composed of octadecylsilane bonded silica gel,0.22%phosphoric acid water as mobile phase A and methanol as mobile phase B with the ratio was 53∶47,the flow rate was set at 1mL·min^(-1),and the detection wavelength was 280nm.RESULTS The peak time of baicalin was about 10 minutes,the peak shape was good and there was no interference from other substances.The content of Baicalin showed a good linear relationship between 0.26 and 1.56μg,and the regression equation was Y=60.999X-1.3235,R^(2)=0.9998.Finally,the content range of Baicalin in Banxia Xiexin Decoction was 309.23~574.28mg.CONCLUSION This method is effective and simple to determine the content of Baicalin in Banxia Xiexin Decoction,which lays a foundation for further research and development of Banxia Xiexin Decoction.

关 键 词：半夏泻心汤 物质基准 黄芩苷 RP-HPLC-UV 

分 类 号：R284.1[医药卫生—中药学]