薄荷-荆芥不同配伍组分体外抗流感病毒作用研究  

Study on Anti-influenza Virus Effect in Vitro of Different Compatibility of Bohe(Menthae Haplocalycis Herba)-Jingjie(Schizonepetae Herba)

作　　者：束雅春[1,2] 王海丹[1] 朱萱萱[1] 孙其然 李伟东[2] 陆兔林[2] SHU Yachun;WANG Haidan;ZHU Xuanxuan;SUN Qiran;LI Weidong;LU Tulin(Affiliated Hospital of Nanjing University of Chinese Medicine,Jiangsu Provincial Hospital of Chinese Medicine,Nanjing 210029,Jiangsu,China;Nanjing University of Chinese Medicine,Nanjing 210046,Jiangsu,China)

机构地区：[1]南京中医药大学附属医院,江苏省中医院,江苏南京210029 [2]南京中医药大学,江苏南京210046

出　　处：《中华中医药学刊》2021年第10期16-20,I0014,I0015,共7页Chinese Archives of Traditional Chinese Medicine

基　　金：国家重点研发计划(2018YFC1707000);国家自然科学基金(81503216);江苏省第五期“333工程”项目(LGY2016017);江苏省六大人才高峰第9批项目(WS-029)。

摘　　要：目的检测薄荷-荆芥不同配伍组分含药血清体外对甲型流感病毒的抑制作用。方法通过含药血清对MDCK细胞毒性的测定,计算含药血清的最大无毒剂量;通过CPE法测定甲型流感病毒TCID_(50),采用Reed-Muench氏法计算甲型流感病毒的TCID_(50),对流感病毒感染性进行测定;再运用MTT法观察薄荷-荆芥不同组分配伍组合含药血清对甲型流感病毒增殖的抑制作用。结果以薄荷挥发油-薄荷黄酮-荆芥黄酮、荆芥挥发油-薄荷黄酮-荆芥黄酮、薄荷挥发油-荆芥挥发油-荆芥黄酮不同比例配伍组分对病毒增殖的抑制作用最为明显。结论薄荷-荆芥不同配伍组分含药血清体外可以抑制流感病毒在细胞内增殖,由此可证实薄荷-荆芥作为核心药对,其不同组分配伍具有确切的抗流感病毒作用。Objective To detect the inhibitory effect of serum containing Bohe(Menthae Haplocalycis Herba)-Jingjie(Schizonepetae Herba) on influenza A virus in vitro. Methods The maximal non-toxic dose was calculated by measuring the cytotoxicity of serum containing drug to MDCK cells. TCID_(50)of influenza A virus was determined by CPE method, and TCID_(50)of influenza A virus was calculated by Reed Muench method. MTT method was used to observe the inhibitory effect of serum containing different compatibility of Bohe(Menthae Haplocalycis Herba)-Jingjie(Schizonepetae Herba) on influenza A virus proliferation. Results The inhibition of virus proliferation was most obvious in the different proportions of volatile oil of Bohe(Menthae Haplocalycis Herba)-flavone of Jingjie(Schizonepetae Herba), volatile oil of Jingjie(Schizonepetae Herba)-flavone of Jingjie(Schizonepetae Herba), volatile oil of Bohe(Menthae Haplocalycis Herba)-flavone of Jingjie(Schizonepetae Herba)-flavone of Jingjie(Schizonepetae Herba). Conclusion The serum containing different compatibility of Bohe(Menthae Haplocalycis Herba)-Jingjie(Schizonepetae Herba) can inhibit the proliferation of influenza virus in vitro, confirming that Bohe(Menthae Haplocalycis Herba)-Jingjie(Schizonepetae Herba) as the core drug pair has definite anti influenza virus effect.

关 键 词：薄荷 荆芥 流感病毒 含药血清 MDCK细胞 MTT法 

分 类 号：R285.5[医药卫生—中药学]