嗜水气单胞菌中转录因子AHA_1581对细菌生理功能调控机制 的研究  

Study on the transcription factor AHA_1581 in Aeromonas hydrophila on the regulation mechanism of bacterial physiological functions

作　　者：李碗芯 赵怡扬 林玲[1,2] 林向民[1,2,3] Wanxin Li;Yiyang Zhao;Ling Lin;Xiangmin Lin(School of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian Province,China;Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian Province,China;Fujian Key Laboratory of Marine Biotechnology,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian Province,China)

机构地区：[1]福建农林大学生命科学学院,福建福州350002 [2]福建农林大学福建省农业生态过程与安全监控重点实验室,福建福州350002 [3]福建农林大学福建省海洋生物技术重点实验室,福建福州350002

出　　处：《微生物学报》2021年第11期3594-3606,共13页Acta Microbiologica Sinica

基　　金：国家自然科学基金(31802343);福建省海洋生物技术重点实验室基金(2020MB04)。

摘　　要：【目的】LuxR家族转录因子能够抑制或刺激不同功能类型基因的表达,来维持细胞功能的稳定性。嗜水气单胞菌是水产养殖中重要的致病菌之一,目前对该菌中的LuxR家族转录因子功能的研究还较少。【方法】本研究利用含有sacB标记的自杀载体pRE112和同源重组技术敲除LuxR家族转录因子AHA_1581基因。【结果】生理表型测定结果发现,ΔAHA_1581的运动与胞外蛋白的酶活增强、生物被膜形成能力降低,且耐受低温、卡那霉素、庆大霉素胁迫,但是对K_(2)Cr_(2)O_(7)更加敏感。进一步对野生型Ah和ΔAHA_1581的定量蛋白质组学分析,共鉴定到2654个蛋白,其中59个蛋白下调表达,142个蛋白上调表达。生物信息学分析表明AHA_1581参与调控双组分调节系统、丙酮酸代谢、碳代谢、TCA循环等细菌重要生理过程,以及细菌耐药基因和毒力因子的差异表达。【结论】了解AHA_1581基因在调控细菌毒力以及生物过程中所起的重要作用,对预防和控制嗜水气单胞菌引起疾病的发生和传播可能具有重要的科学意义。LuxR family transcription factors can inhibit or stimulate the expression of various functional genes to maintain the stability of cell function.[Objective]To study the role of LuxR family transcription factors in Aeromonas hydrophila.[Methods]In this study,we have knocked out the AHA_1581 gene by homologous recombination technology to understand their role in virulence and drug resistance.[Results]The deletion of AHA_1581 gene not only enhanced the extracellular protease enzyme activity,the tolerance of A.hydrophila to survive under low temperature,different antibiotic stress,but also reduced the biofilm formation capability.Further the quantitative proteomic and bioinformatics analysis showed that the deletion of AHA_1581 gene 59 proteins were down-regulated and 142 proteins were up-regulated,which affected several important biological processes and virulence pathways in A.hydrophila.[Conclusion]Overall,the outcome of this study on the role of transcription factor AHA_1581 in the biological processes and virulence of A.hydrophila may pay the way for controlling their infections.

关 键 词：嗜水气单胞菌 LuxR家族转录因子 AHA_1581 定量蛋白质组学 生理功能 

分 类 号：S941.42[农业科学—水产养殖]