球孢白僵菌侵染中黑盲蝽致病过程的电镜观察  

Electron microscopic observation of infection of Adelphocoris suturalis by Beauveria bassiana

作　　者：张啸 刘佳[1] 杨亦桦[1] 武淑文[1] ZHANG Xiao;LIU Jia;YANG Yi-Hua;WU Shu-Wen(College of Plant Protection,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]南京农业大学植物保护学院,南京210095

出　　处：《应用昆虫学报》2021年第5期1190-1198,共9页Chinese Journal of Applied Entomology

摘　　要：【目的】为揭示白僵菌Beauveria bassiana对中黑盲蝽Adelphocoris suturalis的致病机制,并探索盲蝽生物防治的有效方法。【方法】本文利用扫描电子显微镜和透射电子显微镜观察白僵菌菌株Bb-VⅢ对中黑盲蝽成虫的侵染过程。【结果】接种白僵菌后,其分生孢子在中黑盲蝽的体壁褶皱、凹陷处或虫体连接部位附着;接种16h后分生孢子萌发产生芽管,并借助机械压力和酶的作用侵入中黑盲蝽体壁;36-72h时,菌丝体相继侵入中黑盲蝽的肌肉组织、脂肪体和肠体绒毛内,并在昆虫血腔内进行出芽或分隔生殖;接种48h后中黑盲蝽死亡,虫体内的菌丝体伸到体外并萌发成气生菌丝和分生孢子,继而布满虫体。【结论】白僵菌的附着受中黑盲蝽体表物理结构的影响,其分生孢子在接种48 h内完成对中黑盲蝽的寄生并最终导致虫体发病死亡,表现出较强的致病力。菌丝入侵的机械破坏力和酶类的水解作用是白僵菌对中黑盲蝽的重要致病因子。[Objectives]To reveal the pathogenic mechanism of infection of Beauveria bassiana and investigate the effectiveness of B.bassiana as a biological control for mirid bugs.[Methods]The process of infection of Adelphocoris suturalis by the Bb-VⅢstrain of B.bassiana was observed with both scanning and transmission electron microscopy.[Results]At the beginning of inoculation,B.bassiana adhered to epidermal folds,depressions and body joints of A.suturalis.After 16 h germinated conidia formed germ tubes that invaded the cuticle of A.suturalis with the aid of extracellular hydrolyzing enzymes and mechanical stress.After 36-72 h hyphal bodies had invaded muscle tissue,the fat body,intestinal villi,proliferating in the body cavity by both septation and budding.Finally,after 48 h hyphae emerged from the cuticle and developed into aerial hyphae and conidia.The cuticle of dead A.suturalis was completely covered by hyphae.[Conclusion]Attachment of B.bassiana conidia is affected by structural features of the body wall of A.suturalis.The infection process was completed within 48 h of inoculation,which shows that B.bassiana is highly pathogenic to A.suturalis.The mechanism by which hyphae invade the host cuticle involves not only mechanical pressure but also extracellular hydrolase.

关 键 词：白僵菌 中黑盲蝽 透射电子显微镜 扫描电子显微镜 侵染过程 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治]