基于网络药理学探讨实脾饮治疗心力衰竭的作用机制  

Molecular Mechanism Analysis of Heart Failure Treated by Shipin Yin(实脾饮) Based on Network Pharmacology

作　　者：安宜沛 陈彦[1] 孙俊娜 王永霞[1] 陈鹏[1] 胡宇才[1] 于瑞[1] 张孟孟 AN Yipei;CHEN Yan;SUN Junna;WANG Yongxia;CHEN Peng;HU Yucai;YU Rui;ZHANG Mengmeng(The First Affiliated Hospital of Henan University of TCM,Zhengzhou 450000,Henan,China;Henan University of TCM,Zhengzhou 450000,Henan,China)

机构地区：[1]河南中医药大学第一附属医院,河南郑州450000 [2]河南中医药大学,河南郑州450000

出　　处：《实用中医内科杂志》2021年第10期24-27,126,I0019,I0020,F0003,共8页Journal of Practical Traditional Chinese Internal Medicine

基　　金：国家中医药管理局中医药循证能力建设项目(2019XZZX-XXG003);河南省中医药科学研究专项(2018JDZX073)。

摘　　要：目的应用网络药理学探讨中医经典利水方剂实脾饮在治疗心力衰竭(HF)中发挥的多组分、多靶点、多通路的作用机制。方法在BATMAN-TCM数据库中搜索实脾饮,获得其治疗HF的靶蛋白;通过STRING数据库进行蛋白质相互作用分析,应用Metascape对预测的靶蛋白进行富集总分析,通过David 6.8进行GO富集分析、KEGG通路分析及药物-靶点分析。结果经分析,实脾饮中与治疗HF相关预测靶标蛋白共16种。Metascape富集分析显示其靶蛋白主要有参与调节液循环、调节cGMP-PKG信号通路、调控血管收缩的作用。GO富集分析结果显示,实脾饮治疗心衰的可能作用机制中共有39条富集于生物学过程、10条富集于细胞组分、19条富集于分子途径。KEGG通路分析结果显示,实脾饮各组分所参与的信号转导通路中有21条与HF有关。结论实脾饮治疗HF的作用机制主要与其对肾素-血管紧张素、心肌收缩、及心肌细胞离子转运的调节有关,涉及cGMP-PKG信号通路、Ca^(2+)信号通路、cAMP信号通路,主要靶标蛋白为血管紧张素转换酶(ACE)、钠/钾离子转运ATP酶α1亚基(ATP1A1)、肾上腺素能受体(ADRA2A/ADRA2B)、肿瘤坏死因子(TNF)等。Objective To study the mechanism of Shipin Yin(实脾饮) in the treatment of heart failure(HF) based on network pharmacology. Methods We searched Shipi Yinfrom bioinformatics analysis tool for molecular mechanism of traditional Chinese medicine(BATMAN-TCM)(http://bionet.ncpsb.org/batman-tcm/)database, and selected out HF-related target proteins of Shipi Yin to predict enrichment by Metascape and perform online Gene Ontology(GO) enrichment analysis by David 6.8, Kyoto Encyclopedia of Genes andGenomes(KEGG) pathway analysis and drug-target analysis.Results There were 16 main predicted target proteins associated with HF in Shipi Yin. Metascape enrichment analysis predicted that the target protein could regulate blood circulation, cGMP-PKG signaling pathway and vasoconstriction. GO enrichment analysis results showed that a total of 39 were enriched in biologicalprocesses, 10 in cell components, and 19 in molecular pathways.Conclusion The mechanism of Shipi Yin for the treatment of HF is mainly related with the adjustment of renin-angiotensin system and myocardial contraction, as well as the cardiac muscle cells ion transport, involving the cGMP-PKG signaling pathways, calcium signaling pathways and cAMP signaling pathways, and the main target proteins of angiotensin converting enzyme(ACE), sodium/potassium ion transport atpase alpha 1 subunits(ATP1 A1), adrenergic receptor(ADRA2 A/ADRA2 B), tumor necrosis factor(TNF) had important effects through the course of HF.

关 键 词：实脾饮 心力衰竭 网络药理学 靶标蛋白预测 信号通路 

分 类 号：R285[医药卫生—中药学]