健脾清化散瘀饮对慢性萎缩性胃炎模型大鼠胃黏膜TXNDC5、NOX2、NF-κB、TNF-α表达的影响  

Effect of Jianpi Qinghua Sanyu Decoction on Expressions of TXNDC5,NOX2,NF-κB and TNF-α in Mucosa of Rats with Chronic Atrophic Gastritis

作　　者：林翔英 黄铭涵[1] 方文怡[1] 柯晓[1] 高尤亮[1,2] LIN Xiangying;HUANG Minghan;FANG Wengyi;KE Xiao;GAO Youliang(The Second Affiliated Hospital of Fujian University of Traditional Chinese Medicine,Fuzhou 350003,Fujian,China;Ningbo Clinical Pathology Diagnosis Center,Ningbo 315021,Zhejiang,China)

机构地区：[1]福建中医药大学附属第二人民医院,福建福州350003 [2]宁波市临床病理诊断中心,浙江宁波315021

出　　处：《实用中医内科杂志》2021年第10期104-107,I0015,共5页Journal of Practical Traditional Chinese Internal Medicine

基　　金：福建省自然科学基金(2018J01316;2017J01302)。

摘　　要：目的观察健脾清化散瘀饮对慢性萎缩性胃炎(CAG)大鼠胃黏膜炎症水平、萎缩程度,以及含硫氧还蛋白结构域5(TXNDC5)、吞噬细胞NADPH氧化酶(NOX2)、核因子κB(NF-κB)、肿瘤坏死因子-α(TNF-α)表达影响。方法 Wistar清洁级大鼠按照随机数字法分为空白对照组(n=8)与造模组(n=42),采用"氨水+去氧胆酸钠+乙醇法灌胃"法建立CAG大鼠模型,造模成功后,造模组随机分中药低、中、高治疗组,给予相应药物连续干预4周后,采用HE染色检测大鼠胃黏膜病变情况,采用免疫组化法检测胃组织中TXNDC5、NOX2、NF-κB、TNF-α蛋白表达。结果与空白对照组相比,模型组大鼠炎症细胞浸润明显,胃黏膜固有腺体萎缩、减少,胃黏膜组织中TXNDC5、NOX2、NF-κB、TNF-α蛋白表达显著升高(P<0.05或P<0.01)。与模型组比较,健脾清化散瘀饮低、中、高剂量治疗组胃黏膜固有腺体萎缩及炎症程度明显改善,组织中TXNDC5、NOX2、NF-κB、TNF-α蛋白表达水平均有所下降,差异具有统计学意义(P<0.05或P<0.01)。结论健脾清化散瘀饮可有效改善CAG大鼠胃黏膜组织病理改变,其机制可能与降低胃黏膜组织中TXNDC5、NOX2、NF-κB、TNF-α表达有关。Objective To observe the effects of Jianpi Qinghua Sanyu Decoction on inflammation and atrophy of gastric mucosa in rats with chronic atrophic gastritis(CAG), as well as the expressions of TXNDC5, NOX2, NF-κB and TNF-α. Methods Clean-grade wistar rats were divided into blank control group(n=8) and model group(n=42) according to random number method. CAG rats model was established by the method of "ammonia+sodium deoxycholate+ethanol gavage". After successful model building, the model was randomly divided into the esthetic group, the low-dose, medium-dose and high-dose treatment groups of traditional Chinese medicine. After 4 weeks of continuous intervention with corresponding drugs, HE staining was used to detect mucosal lesions, and immunohistochemical method was used to detect protein expressions of TXNDC5, NOX2, NF-κB, and TNF-α in gastric tissue. Results Compared with those of the blank control group, the inflammatory cells in the model group were infiltrated, the proper glands of the gastric mucosa were atrophied and decreased, and the protein expressions of TXNDC5, NOX2, NF-κB and TNF-α were significantly increased(P<0.05 or P<0.01). Compared with those of the model group, the gastric mucosa proper gland atrophy and inflammation in the low-dose, medium-dose and high-dose treatment groups of Jianpi Qinghua Sanyu Decoction were significantly improved, and the expression levels of TXNDC5, NOX2, NF-κB and TNF-α in the tissues were decreased. The difference was statistically significant(P<0.05 or P<0.01). Conclusion The method of Jianpi Qinghua Sanyu Decoction can effectively improve the pathological changes of gastric mucosa in CAG rats, and its mechanism may be related to the reduction of expressions of TXNDC5, NOX2, NF-κB and TNF-α in gastric mucosa.

关 键 词：健脾清化散瘀饮 慢性萎缩性胃炎 胃黏膜病理改变 TXNDC5 NOX2 NF-ΚB TNF-Α 大鼠 

分 类 号：R289.1[医药卫生—方剂学]