伊木萨克提取物及其组合物对复合应激性ED大鼠阴茎组织中ET-1与eNOS、nNOS表达的调控作用研究  

Study on the expression changes of ET-1,eNOS and nNOS in penile tissues of the rats with compound stress type ED and the regulation effect of Yimusake extract and its composition

作　　者：侯鹏程 阿卜杜热伊木江·如则 毛吾兰·买买提依明 刘文娟 斯依提·阿木提 阿地力江·伊明 Hou Pengcheng;Abudureyimujiang Ruze;Maowulan Maimaitiyiming;Liu Wenjuan;Siyiti Amuti;Adilijiang Yiming(Department of Human Anatomy,Collage of Basic Medicine,Xinjiang Medical University,Urumqi,830011,China;Department of Biology,Collage of Basic Medicine,Xinjiang Medical University)

机构地区：[1]新疆医科大学基础医学院人体解剖学教研室,新疆乌鲁木齐830011 [2]新疆医科大学基础医学院生物学教研室

出　　处：《中国男科学杂志》2021年第5期30-36,共7页Chinese Journal of Andrology

基　　金：自治区重点研发计划项目(2016B03044-6)。

摘　　要：目的研究复合应激性ED大鼠模型阴茎组织中内皮素-1(ET-1)内皮型一氧化氮合酶(eNOS)、神经元型一氧化氮合酶(nNOS)表达改变与伊木萨克提取物及其组合物的调控作用,并探讨其生殖生物学意义。方法160只SD雄性大鼠经交配实验确认具有正常性功能后,随机取15只为正常对照(N)组,余145只为造模(M)组,以环境雌激素样饲料联合冷应激复合干预法建立慢性复合应激大鼠模型,造模35周后,通过交配实验从复合应激模型中筛选出ED模型,并随机分为ED模型对照(ED)组、伊木萨克(Yimusake)组、醇提取物(alcohol extract,AE)组、水提取物(water extract,WE)组和提取物组合物(combination of extracts,COE)组,以相应提取物和药物干预3周后,分别采用免疫组化法和免疫印迹法检测阴茎组织中ET-1和eNOS、nNOS的表达。结果(1)免疫组织化学结果:与N组相比,ED组ET-1表达量显著升高(P<0.05),eNOS、nNOS表达量显著降低(P<0.05)。干预后,与ED组相比,ET-1在COE、WE和Yimusake组的表达量均显著降低(P<0.05),同时,COE、WE显著低于伊木萨克组和AE组(P<0.05)。eNOS和nNOS在COE组、AE组、WE和Yimusake组的表达量亦较ED组显著升高(P<0.05),eNOS在各干预组间无显著差异(P>0.05);nNOS在AE和WE组中的表达量均显著低于Yimusake组(P<0.05),在COE组表达明显高于Yimusake组,但无统计学意义(P>0.05)。(2)免疫印迹检测结果:与N组相比,ED组ET-1表达量显著升高(P<0.001),eNOS、nNOS的表达量显著降低(P<0.001)。干预后,ET-1在COE和WE组的表达量均较ED组显著降低(P<0.001),同时COE和WE组的表达量亦显著低于Yimusake组(P<0.001);eNOS表达量在COE、WE、AE和Yimusake组均较ED组显著升高(P<0.001),nNOS表达量在COE、AE和Yimusake组较ED组显著升高(P<0.001);与Yimusake组比较,COE和AE组eNOS、nNOS的表达量均无显著性差异(P>0.05),而WE组则显著低于Yimusake组(P<0.001)。结论(1)该ED大鼠模型的发生与其阴茎组织中ET-1表达升�Objective To study the expressions of endothelin-1(ET-1),endothelial nitric oxide synthase(eNOS)and neuronal nitric oxide synthase(nNOS)in penile tissues of the rats with compound stress type ED,and evaluatethe therapeutic effects of Yimusake extract and its composition,and explore its reproductive biological significance.Methods Total of 160 male SD rats,who were confirmed to have normal sexual function through mating experiment,were randomly divided into normal control group(N,15 rats)and the model group(M,145 rats).The rats in group M received environmental estrogen-like feed combined with cold stress compound intervention and to be a chronic compound stress rat model.After 35 weeks of modeling,ED models were screened from compound stress models through mating experiments and then randomly divided into ED model control group(ED),Yimusake group(Yimusake),alcohol extract group(AE),water extract group(WE),and combination of extracts group(COE).After 3 weeks intervention with the corresponding extracts and drugs,the expressions of ET-1,eNOS and nNOS in penile tissue were detected by immunohistochemistry and western blotting.Results(1)Results of immunohistochemistry:Compared with that in group N,the expression of ET-1 in group ED was significantly increased(P<0.05),whereas the expressions of eNOS and nNOS were significantly decreased(P<0.05).After intervention,compared with that in group ED,the expressions of ET-1 in COE,WE and Yimusake groups were significantly decreased(P<0.05),while its expression in COE and WE groups were significantly lower than that in groupYimusakeand AE(P<0.05).The expressions of eNOS and nNOS in group COE,AE,WE and Yimusake were also significantly higher than those in group ED(P<0.05),but there was no significant difference in eNOS among the intervention groups(P>0.05).The expression of nNOS in group AE and WE were significantly lower than that in group Yimusake(P<0.05),and its expression in group COE was clearly higher than that in group Yimusake,but there was no statistical significance(

关 键 词：勃起功能障碍 内皮素-1 一氧化氮合酶 伊木萨克提取物 复合应激 

分 类 号：R392.8[医药卫生—免疫学] R349.3[医药卫生—基础医学]