SCM-198 ameliorates endometrial inflammation via suppressing the LPS-JNK-cJUN/cFOS-TLR4-NF-κB pathway  

作　　者：Yikong Lin Yunyun Li Xinyi Li Xinhua Liu Xiaolin Wang Min Yu Yizhun Zhu Meirong Du 

机构地区：[1]NHC Key Lab of Reproduction Regulation(Shanghai Institute of Planned Parenthood Research),Hospital of Obstetrics and Gynecology,Fudan University Shanghai Medical College,Shanghai 200011,China [2]State Key Laboratory of Quality Research in Chinese Medicine and School of Pharmacy,Macao University of Science and Technology,Macao 518063,China [3]Shanghai Key Laboratory of Bioactive Small Molecules,Department of Pharmacology,School of Pharmacy,Fudan University,Shanghai 201203,China [4]Shanghai JIAI Genetics and IVF Institute,Obstetrics and Gynecology Hospital of Fudan University,Shanghai 200011,China [5]Obstetrics and Gynecology Hospital of Fudan University,Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases,Shanghai 200011,China

出　　处：《Acta Biochimica et Biophysica Sinica》2021年第9期1207-1215,共9页生物化学与生物物理学报（英文版）

基　　金：the National Basic Research Program of China(No.2017YFC1001403);the National Natural Science Foundation of China(Nos.31970859,81630036,and 91542116);the International Cooperation Project between Macao and Shanghai(No.20410760300);the Strategic Collab-orative Research Program of the Ferring Institute of Reproductive Medicine Supported by Ferring Pharmaceuticals and Chinese Academy of Sciences(No.FIRMX200504);Innovation-oriented Science and Technology Grant from NHC Key Laboratory of Repro-duction Regulation(No.CX2017-2);the Program of Shanghai Academic/Technology Research Leader(No.17XD1400900);Innovative Research Team of High-level Local Universities in Shang-hai and a Key Laboratory Program of the Education Commission of Shanghai Municipality(No.ZDSYS14005).

摘　　要：Endometritis is an inflammatory disease of the endometrium,which is responsible for endometrial dysfunction,decidualization failure,and increased incidence of early pregnancy loss.SCM-198,a synthetic form of leonurine,is well known to possess anti-inflammatory effects.SCM-198 has been reported to display beneficial effects on endometritis.However,the specific mechanisms of SCM-198 in preventing endometritis remain unknown.In this study,we focused on the molecular mechanism of SCM-198 in inhibiting endometritis.The anti-inflammatory effects and the related signaling pathways of SCM-198 were studied in vitro using human endometrial stromal cells(hESCs).Reverse transcriptase-polymerase chain reaction and western blot analysis results demonstrated that SCM-198 markedly inhibited lipopolysaccharide(LPS)-induced endometrial inflammatory response by suppressing the LPS-JNK-cJUN/cFOS-TLR4-NF-κB pathway.The preventive and therapeutic effects of SCM-198 on endometrial inflammation were explored by using a mouse model of LPS-induced endometritis.SCM-198 produced essentially the same effects when administered either post-treatment(after LPS)or pre-treatment(before LPS)via vaginal or intraperitoneal administration.In vivo results indicated that SCM-198 is a potential effective drug for the treatment of endometritis.

关 键 词：SCM-198 ENDOMETRITIS cJUN/cFOS TLR4 NF-κB 

分 类 号：R711.32[医药卫生—妇产科学]