洋金花中生物碱成分的研究  

Alkaloid composition of Datura metel

作　　者：王玉伟 赫军[2] 王晓燕 甘禾[1,2] 张维库 续洁琨[1] WANG Yu-wei;HE Jun;WANG Xiao-yan;GAN He;ZHANG Wei-ku;XU Jie-kun(School of Life Sciences&Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 100029,China;Institute of Clinical Medical Sciences&Department of Pharmacy,China-Japan Friendship Hospital,Beijing 100029,China)

机构地区：[1]北京中医药大学生命科学学院&中药学院,北京100029 [2]中日友好医院临床医学研究所&药学部,北京100029

出　　处：《中草药》2021年第20期6163-6167,共5页Chinese Traditional and Herbal Drugs

基　　金：北京市科委G20工程创新研究(Z181100002218029);国家自然科学基金资助项目(81872761);天然药物活性物质与功能国家重点实验室开放课题(GTZK202001)。

摘　　要：目的研究洋金花(白花曼陀罗Datura metel干燥花)95%乙醇提取物的生物碱成分。方法采用大孔吸附树脂、硅胶、ODS、Sephadex LH-20等柱色谱和反相高效液相色谱方法对洋金花化学成分进行系统分离和纯化,并通过HR-ESI-MS、NMR等波谱学技术鉴定化合物的结构。结果从洋金花乙醇提取物中分离得到13个生物碱类化合物,分别鉴定为3-hydroxy-1-(4-hydroxyphenethyl)pyrrolidine-2,5-dione(1)、L-焦谷氨酸甲酯(2)、喹啉(3)、烟酸(4)、1H-咪唑-4-羧酸丁酯(5)、苯基-β-萘胺(6)、4-(羟甲基)-3-(4-羟基苯乙基)吡啶-2(1H)-酮(7)、半枝莲碱(8)、金色酰胺醇酯(9)、对羟基苯乙胺(10)、苯乙胺(11)、苯丙氨醇(12)、尿苷(13)。结论化合物1~9、11和12为首次从曼陀罗属植物中分离得到,化合物10和13为首次从该植物中分离得到。Objective To study alkaloid from the 95% ethanol extract of the dry flowers from Datura metel. Methods The compounds were systematically isolated and purified by using various chromatographic techniques including macroporous resins, silica gel, ODS and Sephadex LH-20 column chromatographies, as well as reversed phase high-performance liquid chromatography. Their chemical structures were elucidated based on HR-ESI-MS and NMR spectroscopic. Results Thirteen alkaloids compounds were obtained from the dry flowers of D. metel and identified as 3-hydroxy-1-(4-hydroxyphenethyl)pyrrolidine-2,5-dione(1), L-pyroglutamic acid methyl ester(2), quinoline(3), nicotinic acid(4), butyl 1 H-imidazole-4-carboxylate(5), phenyl-β-naphthylamine(6), aristopyridinone A(7), scutebarbatine A(8), aurantiamide acetate(9), tyramine(10), phenethylamine(11), phenyl-alalninol(12) and uridine(13). Conclusion Compounds 1—9, 11 and 12 are found in the genus for the first time. Compound 10 and 13 are isolated from D metel for the first time.

关 键 词：洋金花 白花曼陀罗 生物碱 L-焦谷氨酸甲酯 半枝莲碱 金色酰胺醇酯 苯乙胺 苯丙氨醇 

分 类 号：R284.1[医药卫生—中药学]