基于谱效关系的醋延胡索炮制前后特征成分研究  

Characteristic components in crude and vinegar-processed Corydalis Rhizoma based on relationship between spectrum and effect

作　　者：杜伟锋[1,2] 孙海英[3] 洪浩 朱伟豪 洪智慧 葛卫红[2] 李昌煜[1,4] 张光霁 DU Wei-feng;SUN Hai-ying;HONG Hao;ZHU Wei-hao;HONG Zhi-hui;GE Wei-hong;LI Chang-yu;ZHANG Guang-ji(College of Pharmaceutical Sciences,Zhejiang Chinese Medical University,Hangzhou 311402,China;Research Center of Traditional Chinese Medicine Processing Technology,Zhejiang Chinese Medical University,Hangzhou 311401,China;Taizhou Hospital of Zhejiang Province,Taizhou 317000,China;Academy of Chinese Medical Sciences,Zhejiang Chinese Medical University,Hangzhou 310053,China;College of Basic Medical Sciences,Zhejiang Chinese Medical University,Hangzhou 310053,China)

机构地区：[1]浙江中医药大学药学院,浙江杭州311402 [2]浙江中医药大学中药炮制技术研究中心,浙江杭州311401 [3]浙江省台州医院,浙江台州317000 [4]浙江中医药大学中医药科学院,浙江杭州310053 [5]浙江中医药大学基础医学院,浙江杭州310053

出　　处：《中草药》2021年第20期6178-6187,共10页Chinese Traditional and Herbal Drugs

基　　金：国家重点研发计划—中药饮片质量识别关键技术研究(2018YFC1707001);中医药行业科研专项(201507002);宁波市“科技创新2025”重大专项(20201ZDYF020069);中华中医药学会青年人才托举工程项目(QNRC2-C12)。

摘　　要：目的探讨醋延胡索炮制前后HPLC指纹图谱与镇痛药效的相关性,筛选炮制特征成分。方法建立生延胡索与醋延胡索HPLC指纹图谱,对两者化学成分进行差异性分析,以大鼠扭体反应为模型建立评价指标,采用超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛、孕酮、雌二醇、前列腺素E_(2)(prostaglandin E_(2),PGE_(2))、前列腺素F_(2a)(prostaglandin F_(2a),PGF_(2a))等指标评价镇痛药效,结合灰色关联度分析法与熵权法研究醋延胡索的谱-效关系,指认醋延胡索炮制特征成分。结果建立了不同批次生延胡索饮片、醋延胡索饮片的HPLC指纹图谱,确定了31个共有峰。生延胡索饮片与醋延胡索饮片均有止痛效果,以醋延胡索止痛效果最为显著。镇痛谱效关联度分析得到峰9、18-21、25、26醋炙后关联度得分有了显著的提升,与醋炙后镇痛疗效呈显著正相关。结论通过谱-效关系明确了醋延胡索炮制特征成分为原阿片碱、盐酸巴马汀、盐酸小檗碱、去氢紫堇碱、延胡索乙素、四氢小檗碱、延胡索甲素,为醋延胡索炮制质量控制提供一定依据。Objective To explore the correlation between the HPLC fingerprint of crude and vinegar-processed Yanhusuo(Corydalis Rhizoma) and the analgesic efficacy, and to screen the characteristic components of the processing. Methods The crude and vinegarprocessed Corydalis Rhizoma HPLC fingerprints were established, the differences of chemical composition between the two were analyzed. The evaluation index was established with body torsion reaction model. The analgesic efficacy was evaluated using the superoxide dismutase(SOD), malondialchehyche, progesterone, estradiol, prostaglandin E_(2)(PGE_(2)), prostaglandin F_(2a)(PGF_(2a)) as index,combined with the grey correlation analysis method and entropy weight method to study the refluxing spectrum-effect relationship, so as to identify the chemical composition characteristics of vinegar-processed Corydalis Rhizoma. Results HPLC fingerprints of different batches of crude and vinegar-processed Corydalis Rhizoma slices were established, and 31 common peaks were identified.Both crude and vinegar-processed Corydalis Rhizoma decoction pieces had analgesic effect, and vinegar-processed Corydalis Rhizoma decoction pieces had the most significant analgesic effect. The correlation degree analysis of analgesia spectrum effect showed that the correlation degree scores of peaks 9, 18—21, 25 and 26 were significantly improved after vinegar-processing, and showed a significant positive correlation with the analgesic efficacy after vinegar-processing. Conclusion The characteristic components in the processing of Corydalis Rhizoma were identified as protopine, palmatine hydrochloride, berberine hydrochloride, dehydrocorydaline,tetrahydropalmatine, tetrahydroberberine and corydaline, which provided a certain basis for the quality control of vinegar-processed Corydalis Rhizoma.

关 键 词：醋延胡索 指纹图谱 谱效关系 炮制特征成分 HPLC 超氧化物歧化酶 丙二醛 孕酮 雌二醇 前列腺素E_(2) 前列腺素F_(2a) 原阿片碱 盐酸巴马汀 盐酸小檗碱 去氢紫堇碱 延胡索乙素 四氢小檗碱 延胡索甲素 

分 类 号：R283.1[医药卫生—中药学]