轮状病毒实验室污染检测与常用消毒剂灭活效果研究  

Detection of rotavirus contamination in laboratory and inactivation efficacy of common disinfectant

作　　者：史雯[1] 茅海燕[1] 陆龙喜[1] 孙逸[1] 龚黎明[1] 颜浩 张严峻[1] Shi Wen;Mao Haiyan;Lu Longxi;Sun Yi;Gong Liming;Yan Hao;Zhang Yanjun(Zhejiang Provincial Center for Disease Control and Prevention,Hangzhou 310051,China)

机构地区：[1]浙江省疾病预防控制中心,杭州310051

出　　处：《中华实验和临床病毒学杂志》2021年第5期505-508,共4页Chinese Journal of Experimental and Clinical Virology

基　　金："十三五"国家科技重大专项课题(2018ZX10734401)。

摘　　要：目的了解微生物实验室环境中轮状病毒污染状况,识别实验室内容易发生污染的重点部位,同时评价三种常用消毒剂对轮状病毒的灭活效果。方法用病毒采样拭子对实验操作后的物表、常用仪器、实验室环境以及个人防护用品表面进行采样,并用荧光定量RT-PCR进行轮状病毒核酸检测。同时依照2002年版的《消毒技术规范》分别对含氯消毒剂、乙醇和苯扎氯铵这三种消毒剂进行轮状病毒灭活实验。结果采集标本174份,共检测到13份轮状病毒核酸阳性标本,总体阳性率7.47%。用含有效氯500 mg/L次氯酸钠消毒剂作用5 min,75%的乙醇作用1 min,1500 mg/L苯扎氯铵作用1 min,对轮状病毒的灭活对数值均可达到4.00以上。结论在操作轮状病毒的实验室存在病毒污染的风险,用次氯酸钠、乙醇和苯扎氯铵消毒剂均可有效灭活轮状病毒,可用于轮状病毒去污染。Objective To understand the rotavirus contamination in the environment of microbiology laboratory,identify the key parts prone to contamination in the laboratory,and evaluate the inactivation efficacy of three common disinfectants on rotavirus.Methods The laboratory environment and the surface of personal protective equipment were sampled with virus sampling swab after the experimental operation,and rotavirus nucleic acid was detected by real-time RT-PCR.Rotavirus inactivation experiments were carried out on chlorine containing disinfectant,ethanol and benzalkonium chloride according to the Chinese Technical Standard of Disinfection(2002 version).Results A total of 174 samples were collected and 13 rotavirus nucleic acid positive samples were detected.The overall positive rate was 7.47%.The rotavirus could be inactivated with more than 4.00 log titer reduction,with 500 mg/L sodium hypochlorite disinfectant for 5 minutes,75%ethanol for 1 minute and 1500 mg/L benzalkonium chloride for 1 minute.Conclusions There is a risk of rotavirus contamination in the microbiology laboratory.Sodium hypochlorite,ethanol and benzalkonium chloride disinfectants can effectively inactivate rotavirus and can be used for rotavirus decontamination.

关 键 词：轮状病毒 污染 消毒剂 病毒灭活 

分 类 号：R187[医药卫生—流行病学]