金纳米粒子在细胞中迁移的暗场成像研究  

Research on dark field imaging of Au nanoparticles migration in cell

作　　者：陈丽惠[1,2] CHEN Lihui(Food Engineering College,Zhangzhou Institute of Technology,Zhangzhou 363000;Applied Technology Engineering Center of Fujian University for Further Processing and Safety of Agricultural Products,Zhangzhou 363000)

机构地区：[1]漳州职业技术学院食品工程学院,漳州363000 [2]农产品深加工及安全福建省高校应用技术工程中心,漳州363000

出　　处：《分析试验室》2021年第11期1279-1283,共5页Chinese Journal of Analysis Laboratory

基　　金：漳州市自然科学基金(ZZ2021J49);福建省教育厅中青年教师教育科研项目(JAT171091);福建省现代分离分析科学与技术重点实验室暨污染监测与控制省高校重点实验室开放课题(ST201703);福建省科技计划项目(2018N2002)资助。

摘　　要：采用暗场显微镜观察金纳米粒子在巨噬细胞中的迁移过程,分析其胞吞胞吐机制。巨噬细胞质膜包裹纳米粒子后,其暗场图像的对比度及光散射强度显著增强。基于金纳米粒子特有的光学性质,示踪金纳米粒子由胞吞进入细胞形成囊泡,并在细胞内迁移,孵育90 min后多个囊泡在溶酶体内融合产生纳米粒子聚集体,孵育120 min后,巨噬细胞内吞大量的纳米粒子并融合成多个聚集体。尺寸小的单纳米粒子外排速率大,易于外排出细胞,而纳米粒子聚集体则经过240 min的外排孵育后排出。以纳米粒子作为探针,利用金纳米粒子与细胞在暗场成像中的相互作用,在监测分析生物分子与活细胞的作用机制方面具有广泛的应用前景。Migration of Au nanoparticle( AuNPs) in macrophage was observed by dark field microscopy to analyze the endocytosis and exocytosis. The contrast and scattering intensity of dark field images were significantly enhanced after Au NPs encapsulation in macrophage. Based on the special optical property of AuNPs,their endocytosis was tracked. Vesicle was formed and migrated in the cell. Au NPs aggregation was generated through fusion of several vesicles in lysosome after 90 min culture. More and more AuNPs aggregations were formed after 120 min culture. Exocytosis speed of small Au NPs was fast and easy to be exocytosed from cell. Otherwise,AuNPs aggregations were exocytosed from cell after 240 min. Hence,the concept of interaction between AuNPs and cell in dark field image will be widely applied for monitoring the interaction between biomolecules and live cell in real time with AuNPs as probe.

关 键 词：金纳米粒子 暗场成像 胞吞胞吐 细胞 

分 类 号：O657.3[理学—分析化学]