基于G-四链体-N-甲基卟啉二丙酸的非标记荧光光谱法检测肌氨酸  

A G-quadruplex/N-methyl mesoporphyrin fluorescent probe for label-free detection of sarcosine

作　　者：周斌 胡晞江[1] ZHOU Bin;HU Xijiang(Wuhan Children's Hospital(Wuhan Maternal and Child Healthcare Hospital),Tongji Medical College,Huazhong University of Science&Technology,Wuhan 430014)

机构地区：[1]华中科技大学同济医学院附属武汉儿童医院(武汉市妇幼保健院),武汉430014

出　　处：《分析试验室》2021年第11期1341-1344,共4页Chinese Journal of Analysis Laboratory

基　　金：武汉市卫生健康科研基金(WX19Q04);湖北省卫生健康科研基金(WJ2019H351)项目资助。

摘　　要：核酸适配体与富G序列碱基部分互补配对形成双链DNA。肌氨酸可特异性诱导核酸适配体链发生构象变化,游离出富G序列形成G-四链体,并进一步结合N-甲基卟啉二丙酸(NMM),导致体系荧光强度增加,据此建立了以核酸适配体-富G序列-NMM为荧光探针特异性检测肌氨酸的新方法。在发射波长610 nm处,肌氨酸浓度在9.15~4000 nmol/L范围内时,线性方程为ΔF=0.0551c+1.1256(10^(-7)mol/L),检出限为2.75 nmol/L。该方法用于尿液中肌氨酸含量分析,加标回收率在90.0%~106.7%。对肌氨酸标准溶液测定的相对标准偏差在2.8%~5.6%之间。Theaptamer and the G-rich sequence base are complementarily paired to form double-stranded DNA.Sarcosine can specifically induce conformational changes of the aptamer,thus the G-rich sequence is dissociated to bind with N-methyl mesoporphyrin IX( NMM),resulting in an increase of fluorescence intensity. A novel method for the specific determination of sarcosinewas was successfully established using aptamer-G-rich sequences as fluorescent probes. At the emission wavelength of 610 nm, a linear equation between the fluorenscence intensity and sarcosine concentration was ΔF = 0. 0551 c + 1. 1256( 10^(-7)mol/L) in the range of 9. 15-4000 nmol/L with the detection limit of 2. 75 nmol/L. This method can be applied to the determination of sarcosine in urine. The recovery rates were 90. 0%-106. 7%. The relative standard deviations of the determination of sarcosine were between 2. 8% and 5. 6%.

关 键 词：核酸适配体 肌氨酸 G-四链体 荧光光谱法 

分 类 号：O657.39[理学—分析化学]