蝉蜕水煎剂对妊娠晚期大鼠子宫运动节律及PGE_(2)、COX-2水平的影响  

Effect of Cicada Slough Decoction on Uterine Motility Rhythm of Rats in Third Trimester of Pregnancy and Its Influences to Levels of PGE_(2) and COX-2

作　　者：王锋[1] 徐静[1] 童婷婷 崔利娜[1] 付秀虹[1] WANG Feng;XU Jing;TONG Tingting;CUI Lina;FU Xiuhong(Luohe Central Hospital,Luohe 462000,China)

机构地区：[1]漯河市中心医院,河南漯河462000

出　　处：《中医药信息》2021年第11期27-32,共6页Information on Traditional Chinese Medicine

基　　金：河南省医学科技攻关计划项目(2018021025)。

摘　　要：目的:探讨蝉蜕水煎剂对妊娠晚期大鼠子宫运动节律及前列腺素E_(2)(PGE_(2))、环氧化酶-2(COX-2)水平的影响。方法:100只妊娠晚期SD大鼠随机分为模型组、生理盐水组(安慰组)、蝉蜕水煎剂低剂量组(中低组)、蝉蜕水煎剂高剂量组(中高组)和阳性对照组(阳性组),每组20只。经干预后,观察大鼠分娩启动时间和产程时间;部分大鼠脱颈处死,取完整子宫检测子宫收缩强度、频率及子宫收缩力;HE染色法观察子宫宫颈组织形态;ELISA法检测大鼠血清雌二醇(E_(2))、孕酮(P)水平;qRT-PCR与WesternBlot法检测大鼠子宫组织中PGE_(2)和COX-2的mRNA与蛋白相对表达水平。结果:模型组与安慰组大鼠所有检测指标差异均无统计学意义(P>0.05);与模型组比较,各药物组大鼠分娩启动时间与总产程时间较短,宫缩频率较高,宫缩强度及宫缩力较大(P<0.05),且中低组、中高组、阳性组以上指标显著程度依次递增(P<0.05);与模型组比较,中低组、中高组、阳性组宫颈结缔组织与胶原纤维排列紊乱、疏松,间质水肿明显;与模型组比较,各药物组大鼠血清E_(2)水平较高,P水平较低(P<0.05),且中低组、中高组、阳性组以上指标显著程度依次递增(P<0.05)。与模型组比较,各药物组大鼠子宫组织中COX-2和PGE_(2)的mRNA与蛋白表达水平较高(P<0.05),且中低组、中高组以上指标显著程度依次递增(P<0.05)。结论:蝉蜕水煎剂可改善妊娠晚期大鼠子宫运动节律,上调子宫组织PGE_(2)、COX-2表达水平。Objective:To observe the effect of cicada slough decoction on the uterine movement rhythm of rats in third trimester of pregnancy and its influence to the levels of prostaglandin E_(2)(PGE_(2))and cyclooxygenase 2(COX-2).Methods:100 SD rats in third trimester of pregnancy were randomly divided into the model group,the normal saline group(the placebo group),the cicada slough decoction groups of low-medium dose group,medium-high dose and the positive control group,with 20 rats in each group.After a series of interventions,the start time of labor and the duration of labor were observed in the rats.Part of the rats were sacrificed by cervical dislocation,the whole uterus was taken by laparotomy,and the intensity,frequency and force of uterine contraction were measured.The morphology of uterine cervix was observed by HE staining;the levels of estradiol(E_(2))and progesterone(P)were detected by ELISA;the mRNA and protein expression levels of PGE_(2) and COX-2 of uterine tissues were detected by qRT-PCR and Western blot.Results:There were no statistical differences in the initial time of labor,labor duration,uterine contraction intensity,uterine contraction frequency,uterine contraction force,uterine morphology,serum levels of E_(2) and P,mRNA and protein expressions of PGE_(2) and COX-2 in the uterine tissues between the model group and the placebo group(P>0.05).Compared to those in the model group,the initial time of labour and the total labor duration were shorter,the frequency of contractions was higher,and the intensity and force of contractions were larger in all medication groups(P<0.05);of which the improvements were increased in the order of the low-medium dose group,medium-high dose group and the positive group.Compared to those in the model group,the cervical connective tissues and collagen fibers were arranged disorderly and loosely and the interstitial edema was obvious in the low-medium dose group,medium-high dose group and the positive group.The serum level of E_(2) was higher,and the serum level of P was

关 键 词：蝉蜕 妊娠晚期 子宫运动节律 前列腺素E_(2) 环氧化酶-2 

分 类 号：R285.5[医药卫生—中药学]