奥美沙坦对大鼠树突细胞抗原递呈功能作用的研究  

The effect of olmesartan on the antigen presentation function of rat dendritic cells

作　　者：毕延萍[1] 王聪聪[2] 李亨[2] 张民[2] 杨春林 李晓丽[2] 段瑞生[2,3] Bi Yanping;Wang Congcong;Li Heng;Zhang Min;Yang Chunlin;Li Xiaoli;Duan Ruisheng(Department of Emergency Medicine,the First Affiliated Hospital of Shandong First Medical University&Shandong Provincial Qianfoshan Hospital,Jinan 250014,China;Shandong Provincial Qianfoshan Hospital,Jinan 250014,China;Shandong Institute of Neuroimmunology,Jinan 250014,China;Shandong Provincial Key Laboratory for Rheumatic Disease and Translational medicine,Jinan 250014,China)

机构地区：[1]山东第一医科大学第一附属医院(山东省千佛山医院)急诊医学科,济南250014 [2]山东第一医科大学第一附属医院(山东省千佛山医院)神经内科山东省神经免疫研究所,济南250014 [3]山东省风湿免疫病转化医学重点实验室,济南250014

出　　处：《中华急诊医学杂志》2021年第11期1324-1328,共5页Chinese Journal of Emergency Medicine

基　　金：山东省医药卫生科技发展计划(2017WS082);山东第一医科大学学术提升计划(2019QL013)。

摘　　要：目的探讨奥美沙坦对大鼠树突细胞(dendritic cells,DCs)抗原递呈功能的影响。方法取雌性Lewis大鼠髓源DCs,加入奥美沙坦(终浓度10μmol/L)和等体积的DMSO,分别记为奥美沙坦修饰的DCs(olmesartan modified dendritic cells,OLM-DCs)和未经奥美沙坦修饰的DCs(control DCs,Con-DCs)。使用流式细胞仪分别测定DCs表面CD80、CD86和MHCⅡ平均荧光强度(mean fluorescence intensity,MFI)及DCs中IL-10和TGF-β的表达。OLM-DCs和Con-DCs分别与卵白蛋白(OVA)致敏的淋巴结CD4+T细胞共培养,流式细胞仪检测T淋巴细胞增殖,ELISA检测细胞培养上清液中IFN-γ、IL-10水平。结果奥美沙坦抑制了DCs表面MHCⅡ分子的表达,促进了IL-10的产生。与Con-DCs组比较,OLM-DCs抑制了T淋巴细胞增殖反应和IFN-γ的产生。结论在体外,奥美沙坦能够诱导产生耐受性DCs,后者能够抑制T淋巴细胞增殖、抑制Th1细胞因子的水平。Objective To explore the effect of Olmesartan on the antigen presenting function of dendritic cells(DCs)in rats.Methods Bone marrow-derived dendritic cells of female Lewis rats were obtained.Bone marrow-derived dendritic cells were cultured with Olmesartan(final concentration 10μmol/L;OLM-DCs)or equal volume of DMSO(Con-DCs).Mean fluorescence intensity(MFI)of the surface costimulatory molecule CD80,CD86 and MHCⅡon DCs and the levels of IL-10 and TGF-βof DCs were analyzed by flow cytometry.DCs and CD4+T lymphocytes were cocultured.T lymphocytes proliferation was analyzed by flow cytometry.IFN-γin the supernatants were determined by ELISA.Results The expression of MHCⅡon DCs was inhibited by Olmesartan.The level of intracellular IL-10 in DCs was up-regulated by Olmesartan.Compared with Con-DCs,T lymphocytes proliferation and the level of IFN-γwere inhibited by OLM-DCs.Conclusions Olmesartan could induce tolerogenic DCs in vitro.These tolerogenic DCs could inhibit T lymphocytes proliferation and the production of Th1 cytokine.

关 键 词：奥美沙坦 树突细胞 T淋巴细胞 动脉粥样硬化 免疫炎症 

分 类 号：R543.5[医药卫生—心血管疾病]