孕中期肠道菌群特征及其与妊娠期糖尿病的关系  

Association of characteristics of gut microbiome with gestational diabetes mellitus in pregnant women during the second trimester of pregnancy

作　　者：苏尧 陈莉[1] 徐先明[1] SU Yao;CHEN Li;XU Xianming(Department of Obstetrics and Gynecology,Shanghai General Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 201600,China)

机构地区：[1]上海交通大学附属上海市第一人民医院妇产科上海交通大学医学院,上海201600

出　　处：《实用医学杂志》2021年第20期2636-2640,2646,共6页The Journal of Practical Medicine

基　　金：上海申康医院发展中心临床三年行动计划资助(编号:SHDC2020CR2060B)。

摘　　要：目的分析妊娠期糖尿病(GDM)和妊娠期糖耐量正常(GNGT)孕妇肠道菌群特征及与GDM关系。方法选取2019-2020年在我院产检的42名孕中期孕妇为研究对象,行OGTT当天采集新鲜粪便,利用IlluminaHiseq 2500平台检测细菌16SrRNA的V3-V4区,并进行生信分析。结果(1)门属水平上,两组孕妇肠道菌群构成存在差异,GDM组OTUs和多样性较GNGT组高。(2)拟杆菌门和属、厚壁菌门、放线菌门和瘤胃菌属-UCG014与糖代谢水平有相关性。(3)拟杆菌门和菌属作为预测GDM的标志物的敏感性和特异性达70%~80%。结论GDM孕妇和GNGT孕妇肠道菌群构成不同并与糖代谢指标具有相关性;GDM孕妇丰度明显增高的拟杆菌门和属可期望为预测GDM的因子。Objective To analyze the characteristics of gut microbiome in pregnant women with gestational diabetes mellitus(GDM)and gestational normal glucose tolerance(GNGT)during the second trimester and its relationship with GDM.Methods A total of 42 pregnant women in the second trimester receiving obstetric examination in our hospital from 2019 to 2020 were selected.Fresh feces were collected on the day of OGTT test.DNA sequencing was performed on the V3-V4 variable region of bacterial 16 SrRNA using IlluminaHiSeq 2500 platform,and bioinformatics analysis was performed.Results At the phylum level,there were differences in intestinal flora composition between the two groups,and the OTUs and diversity of GDM group were higher than that of GNGT group.Bacteroidetes and genera,Firmicutes,Actinomycetes and rumen bacterium genera-UCG014 were correlated with glucose metabolism.The sensitivity and specificity of Bacteroidetes and Bacteria as markers for predicting GDM reached 70%~80%.Conclusions The composition and diversity of intestinal flora in GDM women and GNGT women during the middle trimester of pregnancy were different;the intestinal flora in the middle trimester of pregnancy was correlated with the glucose metabolism index,and Bacteroidetes and Bacteroides could be used as a marker for predicting GDM.

关 键 词：肠道菌群 妊娠期糖尿病 多样性 

分 类 号：R714.7[医药卫生—妇产科学]