DKK-1基因启动子甲基化与强直性脊柱炎病理性成骨的关系  

Relationship between DKK-1 gene promoter methylation and pathological osteogenesis in Ankylosing Spondylitis

作　　者：陈慧 吴娟 邹宇聪 CHEN Hui;WU Juan;ZOU Yucong(Department of Rehabilitation,the Third Affiliated Hospital of Southern Medical University,Guangzhou 510630,China;不详)

机构地区：[1]南方医科大学第三附属医院康复科,广州510630

出　　处：《实用医学杂志》2021年第20期2692-2695,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金青年基金(编号:81703883)。

摘　　要：目的探讨DKK-1基因启动子甲基化与强直性脊柱炎(ankylosing Spondylitis,AS)的病理性成骨关系。方法选择2017年7月至2020年1月于南方医科大学第三附属医院就诊的强直性脊柱炎患者4例(AS组)和外伤致股骨颈骨折患者4例(FNF组)为研究对象,Western Blot检测DKK-1表达,MSP检测DKK-1甲基化状态,qPCR检测DNMTs。结果AS组较FNF组,DKK-1明显降低(P<0.05),DKK-1基因启动子甲基化水平明显升高(P<0.05),而DNMTs两组比较差异无统计学意义(P>0.05)。结论DKK-1基因可能通过高甲基化抑制DKK-1表达促进AS的病理性成骨,可用于评估AS的病理性成骨发展,为AS的治疗提供线索。Objective To investigate the relationship between DKK-1 gene promoter methylation and pathological osteogenesis in Ankylosing Spondylitis(AS).Methods Western Blot was used to detect the expression of DKK-1 protein in hip joint capsule tissue from 4 patients with AS and 4 with femoral neck fracture(FNF)caused by trauma in the Third Affiliated Hospital of Southern Medical University.DKK-1 gene promoter methylation was examined by Methylation-specific PCR(MSP).Meanwhile,DNA methyltransferases(DNMTs)were evaluated by Quantitative Real-time polymerase chain reaction(qPCR).Results The expression of DKK-1 protein in AS hip joint capsule tissue was significantly lower than that in FNF group(P<0.05),while the methylation status of DKK-1 gene promoter was significantly higher(P<0.05).And there was no significant difference about DNMTs between two groups.Conclusion Lower DKK-1 promotes the pathological osteogenesis of AS through DKK-1 gene promoter hypermethylation,which can be used to evaluate the development of AS pathological osteogenesis and provide clues for the treatment of AS.

关 键 词：强直性脊柱炎 DKK-1 DNA甲基化 DNA甲基转移酶 

分 类 号：R593.23[医药卫生—内科学]