高原鼢鼠阴道涂片HE、瑞氏和快速革兰氏染色法比较  

Comparison of hematoxylin-eosin,Wright and rapid Gram staining methods of plateau zokor vagina smear(Eospalax baileyi)

作　　者：王艳莉 姚宝辉 谭宇尘 康宇坤 张德罡[1,2] 苏军虎[1,2] WANG Yan-li;YAO Bao-hui;TAN Yu-chen;KANG Yu-kun;ZHANG De-gang;SU Jun-hu(College of Pratacultural Science,Gansu Agricultural University/Key Laboratory of Grassland Ecosystem,Ministry of Education/Pratacultural Engineering Laboratory of Gansu Province/Sino-U.S.Centers for Grazingland Ecosystem Sustainability,Lanzhou 730070,China;Gansu Agricultural University-Massey University Research Centre for Grassland Biodiversity,Gansu Agricultural University,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学草业学院,草业生态系统教育部重点实验室/甘肃省草业工程实验室,中-美草地畜牧业可持续发展研究中心,甘肃兰州730070 [2]甘肃农业大学-新西兰梅西大学草地生物多样性研究中心,甘肃兰州730070

出　　处：《草原与草坪》2021年第5期119-124,共6页Grassland and Turf

基　　金：国家自然科学基金项目(31760706);甘肃省陇原青年创新创业人才项目(LYRC2018-02)。

摘　　要：采用HE、瑞氏和快速革兰氏3种染色方法,比较了高原鼢鼠(Eospalax baileyi)阴道涂片的染色背景、过程和效果,以筛选最佳染色方法。结果显示:HE染色后涂片背景及细胞分型清晰,核质着色差异明显且检出率高,与其他两种方法的检出率差异显著(P<0.01);瑞氏染色核质呈不同程度的蓝紫色,背景杂乱,核质染色差别不明显;快速革兰氏染色将细胞核、细胞质分别染成紫红色和玫红色,背景相对暗淡,白细胞则染成深红色,核质着色不易区分,影响观察效果。综合分析,HE染色能准确、高效地鉴别高原鼢鼠的发情周期,是高原鼢鼠较理想的一种阴道涂片染色方法。This study used three staining methods including Hematoxylin-eosin(HE),Wright,and Rapid Gram to compare the staining background,process and effect of plateau zokor(Eospalax baileyi)vaginal smears to screen the best staining method.The results showed that HE staining produced clear smear background and cell types with the nuclear and cytoplasmic staining being significantly different,and high detection rate which was statistically different from the other two methods(P<0.01).Nucleocytoplasmic staining by Wright was blue-purple in varying degrees with messy background,and the difference between nuclear and cytoplasmic staining was not obvious.Rapid Gram staining stained the nucleus and cytoplasm in purple and rose red,respectively,with a relatively dark background,while leukocytes were stained in deep red,with nucleus and cytoplasm not easily distinguished.In conclusion,HE staining could accurately and efficiently identify the estrus cycle of plateau zokor;therefore it is an ideal method for staining vaginal smears.

关 键 词：瑞氏染色 HE染色 快速革兰氏染色 阴道涂片 高原鼢鼠 

分 类 号：S812.6[农业科学—草业科学]