MicroRNA is a potential target for therapies to improve the physiological function of skeletal muscle after trauma  

作　　者：Xin-Yi Gu Bo Jin Zhi-Dan Qi Xiao-Feng Yin 

机构地区：[1]Department of Orthopedics and Traumatology,Peking University People’s Hospital,Beijing,China [2]Key Laboratory of Trauma and Neural Regeneration(Peking University),Beijing,China [3]Department of Orthopedics,The First Affiliated Hospital of Nanjing Medical University,Jiangsu Province Hospital,Nanjing,Jiangsu Province,China

出　　处：《Neural Regeneration Research》2022年第7期1617-1622,共6页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China,Nos.82072162,81971177;Beijing Municipal Natural Science Foundation of China,No.7192215(all to XFY)。

摘　　要：MicroRNAs can regulate the function of ion channels in many organs.Based on our previous study we propose that miR-142a-39,which is highly expressed in denervated skeletal muscle,might affect cell excitability through similar mechanisms.In this study,we overexpressed or knocked down miR-142a-3p in C2C12 cells using a lentivirus method.After 7 days of differentiation culture,whole-cell currents were recorded.The results showed that overexpression of miR-142a-3p reduced the cell membrane capacitance,increased potassium current density and decreased calcium current density.Knockdown of miR-142a-3p reduced sodium ion channel current density.The results showed that change in miR-142a-3p expression affected the ion channel currents in C2C12 cells,suggesting its possible roles in muscle cell electrophysiology.This study was approved by the Animal Ethics Committee of Peking University in July 2020(approval No.LA2017128).

关 键 词：C2C12 DENERVATION ion channels MICRORNA miR-142a-3p muscle patch clamp potassium sodium whole-cell currents 

分 类 号：R446[医药卫生—诊断学] R741[医药卫生—临床医学]