Relationships between Diatom Abundances in Rat Organs and in Environmental Waters  

作　　者：Liang REN Yi-qun CHEN Qian-li ZHOU Zun-zhong LIU Ying LI Quan LIU 

机构地区：[1]Department of Forensic Medicine,Tongji Medical College,Huazhon^University of Science and Technology,Wuhan 430030,China [2]Hubei Key Laboratory of Forensic Science,Hubei University of Police,Wuhan 430034,China [3]Guangdong Tongji Forensic Science Institute,Foshan 516500,China

出　　处：《Current Medical Science》2021年第5期981-986,共6页当代医学科学（英文）

基　　金：supported by the Hubei Provincial Natural Science Foundation of China(No.2019CFC861).

摘　　要：Objective:A diagnosis of drowning remains one of the most challenging issues in forensic science,especially for decomposed bodies.Diatom analysis is considered as an encouraging method for diagnosing drowning.In this study,we developed a drowned rat model using different diatom densities in water.Methods:A total of 120 adult Sprague-Dawley rats were used and divided into six groups,wherein experimental groups 1-5 were drowned rats(group A)and postmortem submersion rats(group B)that were submerged in water with five different Cyclotella sp.diatom densities,while the remaining group was used as a blank control.The combination of microwave digestion and vacuum filtration method was used to accomplish efficient tissue digestion and ascertain higher accuracy of diatom determinations within organs.Results:The abundances of diatoms in the lungs,livers,and kidneys were significantly different.The diatom abundances in the lungs,livers,and kidneys were directly proportional to the water diatom densities,and specific quantitative relationships could be approximated by separate regression equations for each organ type.However,the trends associated with the diatom increases among organs slightly differed.In addition,the diatom abundances in the lungs,livers,and kidneys were all positively correlated.Diatoms were not observed in the postmortem submersion groups nor in the blank control groups.Conclusion:The results of this study provide valuable information for establishing a quantitative diatom framework for informing future forensic medicine efforts.

关 键 词：DROWNING diatom test quantitative analysis lungs LIVERS KIDNEYS vacuum filtration 

分 类 号：Q94[生物学—植物学]