一种新的染色方法在植物石蜡切片中的应用  

A New Dyeing Method Applied to Plant Paraffin Sections

作　　者：陆锦鸿 卢衍琳 赵赣[1] 张少翃[2] Lu Jinhong;Lu Yanlin;Zhao Gan;Zhang Shaohong(College of Life Science South China Agricultural University,Guanzhou 510642;Experimental Basis and Practical Training Center South China Agricultural University,Guanzhou 510642,China)

机构地区：[1]华南农业大学生命科学学院,广东广州510642 [2]华南农业大学基础实验与实践训练中心,广东广州510642

出　　处：《广东化工》2021年第19期28-28,31,共2页Guangdong Chemical Industry

基　　金：省级大学生创新创业训练计划(201910564158)。

摘　　要：为了解决植物石蜡切片制作过程中染色步骤繁琐、优片率低下的问题,本研究以水稻为材料探索了石蜡切片制作过程中新的染色方法。新的染色方法将材料整体染色后再进行切片,成片效果与原工艺没有差异,但试剂耗材损耗下降,优片率显著上升。新的染色方法利用叔丁醇替代了二甲苯脱水的作用,叔丁醇脱水效果好且低毒,达到了绿色化学的目的。本研究为石蜡切片制作工业改良提供了参考。The new low-absorption method accompanies the material as a whole with experience,and the new low-absorption method accompanies the material as a whole.This study explores new dyeing methods in the paraffin absorption process for philosophical purposes.The new attack method uses tert-butanol to replace the effect of the second extraction.Tert-butanol has a good extraction effect and low toxicity,achieving the goal of green chemistry.This research is made for the paraffin industry.Growth provides a reference.

关 键 词：植物学 石蜡切片 组织染色 工艺改良 

分 类 号：TQ[化学工程]