黄芪多糖对脓毒症急性肾损伤大鼠AMPK/SIRT1信号通路介导的肾上皮细胞能量代谢的影响  

Effects of astragalus polysaccharides on energy metabolism of renal epithelial cells mediated by AMPK/SIRT1 signaling pathway in rats with septic acute kidney injury

作　　者：王旭东 赵玉良 史为涛 赵云 刘雅文 刘妍 王晴 李智 吴苏明 WANG Xu-dong;ZHAO Yu-liang;SHI Wei-tao;ZHAO Yun;LIU Ya-wen;LIU Yan;WANG Qing;LI Zhi;WU Su-ming(Department of Critical Care Medicine,First Municipal People's Hospital/Affiliated Municipal Hospital,Xuzhou Medical University,Jiangsu Xuzhou 221116,China)

机构地区：[1]徐州市第一人民医院/徐州医科大学附属徐州市立医院重症医学科,江苏徐州221116

出　　处：《中国医院药学杂志》2021年第21期2181-2185,共5页Chinese Journal of Hospital Pharmacy

基　　金：江苏省高校自然科学研究项目(编号:12KJD320005)。

摘　　要：目的:研究黄芪多糖(APS)对脓毒症急性肾损伤(AKI)大鼠肾上皮细胞能量代谢的影响。方法:60只大鼠随机抽取10只设为正常组,其余随机分为AKI组,APS低、高剂量组各10只,地塞米松组11只。建模后6,12,18 h时APS低、高剂量组APS 100,200 mg·kg^(-1)灌胃,地塞米松组地塞米松10 mg·kg^(-1)灌胃,正常组及AKI组等量生理盐水灌胃。建模后24 h测磷法检测肾上皮细胞钠-钾-三磷酸腺苷酶(Na^(+)-K^(+)-ATP)活性;Western blot法检测肾组织沉默信息调节因子2相关酶类1(SIRT1)。结果:与AKI组比较,APS低、高剂量组,地塞米松组Na^(+)-K^(+)-ATP酶活性增强(t=3.894,P=0.005;t=6.564,P<0.01;t=6.565,P<0.01);与APS低剂量组比较,APS高剂量组、地塞米松组Na^(+)-K^(+)-ATP酶活性增强(t=2.544,P=0.034;t=2.635,P=0.030);与AKI组比较,APS低、高剂量组,地塞米松组SIRT1蛋白表达量升高(t=9.923,P<0.01;t=12.042,P<0.01;t=10.960,P<0.01);与APS低剂量组比较,APS高剂量组、地塞米松组SIRT1蛋白表达量升高(t=4.985,P=0.001;t=5.000,P=0.001)。结论:APS可改善脓毒症AKI大鼠肾上皮细胞能量代谢,可能与激活AMPK/SIRT1信号通路有关。OBJECTIVE To explore the effect of astragalus polysaccharide(APS)on energy metabolism of renal epithelial cells in rats with septic acute kidney injury(AKI).METHODS Ten of 60 rats were randomly selected as normal group.The remainder were randomly divided into AKI,APS low/high dose groups(n=10 each)and dexamethasone group(n=11).At 6/12/18 h post-modeling,APS low/high dose groups received 100/200 mg·kg^(-1) intragastrically,dexamethasone group 10 mg·kg^(-1) intragastrically while normal and AKI groups the same amount of normal saline gavage.Phosphorus was measured at 24 h post-modeling for detecting sodium-potassium-adenosine triphosphate(Na^(+)-K^(+)-ATP)activities in renal epithelial cells.Western blot was utilized for detecting the relative protein expression of renal tissue silence information regulator 2 related enzymes 1(SIRT1).RESULTS As compared with AKI group,the Na^(+)-K^(+)-ATPase activities of APS low/high dose and dexamethasone groups were stronger(t=3.894,P=0.005;t=6.564,P<0.01;t=6.565,P<0.01).As compared with APS low-dose group,the Na^(+)-K^(+)-ATPase activities of APS low/high dose and dexamethasone groups were stronger(t=2.544,P=0.034;t=2.635,P=0.030).As compared with AKI group,the relative protein expression of SIRT1 were higher in APS low/high dose and dexamethasone groups(t=9.923,P<0.01;t=12.042,P<0.01;t=10.960,P<0.01).As compared with PS low-dose group,the relative protein expression of SIRT1 was higher in APS high-dose and dexamethasone groups(t=4.985,P=0.001;t=5.000,P=0.001).CONCLUSION APS can improve energy metabolism of renal epithelial cells in septic AKI rats.It may be correlated with an activation of AMPK/SIRT1 signaling pathway.

关 键 词：脓毒症急性肾损伤 黄芪多糖 肾上皮细胞 能量代谢 5-单磷酸腺苷活化蛋白激酶 沉默信息调节因子2相关酶类1 

分 类 号：R631.2[医药卫生—外科学]