益肾达络饮对NMO-IgG作用的视神经脊髓炎大鼠星形胶质细胞的作用及机制研究  

Effect and mechanism of Yishen Daluo Decoction on astrocytes in rats with NMO IgG induced neuromyelitis optica

作　　者：李迎霞[1] 关东升[1] 郭健[2] 任德启[2] LI Ying-xia;GUAN Dong-sheng;GUO Jian;REN De-qi(Henan University of Chinese Medicine,Zhengzhou Henan 450046,China;Henan Province Hospital of TCM,Zhengzhou Henan 450002,China)

机构地区：[1]河南中医药大学,河南郑州450046 [2]河南省中医院,河南郑州450002

出　　处：《时珍国医国药》2021年第8期1881-1883,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(81673943)。

摘　　要：目的观察视神经脊髓炎大鼠星形胶质细胞NMO-IgG的毒性作用,探讨益肾达络饮对其治疗的作用机制。方法原代培养大鼠星形胶质细胞,用免疫荧光法检测AQP4的含量、Western-blot检测AQP4的表达、LDH实验检测NMO-IgG的毒性,以及加入益肾达络饮后AQP4的表达变化。结果免疫荧光法检测AQP4的含量在NMO-IgG组的含量增加,细胞发生变性;Western-blot检测AQP4的表达在NMO-IgG组表达增强,LDH毒性实验显示NMO-IgG的毒性增强。当加入益肾达络饮后NMO-IgG组AQP4的含量降低,表达减弱,NMO-IgG的毒性降低。结论益肾达络饮对NMOSD大鼠星形胶质细胞中的保护作用主要是通过阻断NMO-IgG特异性的结合AQP4,减弱星形胶质细胞的毒性,修复神经细胞的损伤。Objective To observe the toxic effect of NMO-IgG on astrocytes in rats with optic neuromyelitis and to explore the mechanism of Yishendaoluo decoction on its treatment.Methods The content of AQP4,the expression of AQP4 by Western-blot,LDH assay was used to detect the toxicity of NMO-IgG and the expression of AQP4 by adding Yishendaluo decoction were detected by immunofluorescence method in primary cultured rat astrocyte.Results The content of AQP4 was increased in NMO-IgG group,the expression of AQP4 was increased in NMO-IgG group by Western-blot,and the toxicity of NMO-IgG was increased by LDH.The content and expression of AQP4 in NMO-IgG group decreased and the toxicity of NMO-IgG decreased when Yishendaluo decoction was added.Conclusion The protective effect of Yishendaluo Yin on NMOSD rat astrocyte is mainly by blocking NMO-IgG binding to AQP4,attenuating the toxicity of astrocyte and repairing the injury of neural cells.

关 键 词：益肾达络饮 视神经脊髓炎 NMO-IGG 星形胶质细胞 

分 类 号：R285.5[医药卫生—中药学]