Vero细胞悬浮驯化及增殖鸡传染性支气管炎病毒工艺研究  

Domestication and proliferation of avian infectious bronchitis virus in Vero cell suspension culture

作　　者：程旭[1] 姜逸[1] 高明燕[1] 周生[1] 俞燕[1] 李建梅[1] 沈欣悦[1] 范建华[1] Cheng Xu;Jiang Yi;Gao Mingyan;Zhou Sheng;Yu Yan;Li Jianmei;Shen Xinyue;Fan Jianhua(Institute of Poultry,Chinese Academy of Agricultural Sciences,Jiangsu Yangzhou 225125)

机构地区：[1]中国农业科学院家禽研究所,江苏扬州225125

出　　处：《现代畜牧兽医》2021年第11期1-3,共3页Modern Journal of Animal Husbandry and Veterinary Medicine

基　　金：扬州市科技计划(现代农业)项目(YZ2020052);江苏省自然科学基金面上项目(BK20201483)。

摘　　要：细胞苗因工艺优良、产品性质稳定等特点,一直是禽苗研究的主要方向。研究利用QX型传染性支气管炎毒株MJ株感染生物反应器中悬浮培养的Vero细胞,确定培养参数。结果表明,微载体浓度10 g/L、10%DMEM培养基、细胞与微载体比例为50∶1时,细胞生长状态良好。MJ株与Vero细胞感染复数为0.50时,病毒扩增效率最高,在108 h病毒含量可达到10^(6.53) TCID_(50)/mL。Cell vaccine has been the main research direction of poultry vaccine because of its excellent technology and stable product properties.In the study,QX infectious bronchitis virus MJ strain adapted to Vero cells was used to infect Vero cells suspended in bioreactor to determine the culture parameters.The results showed that when the concentration of microcarrier was 10 g/L,10%DMEM medium and the ratio of cell to microcarrier was 50∶1,the cells grew well.The infection complex of MJ strain and Vero cell was 0.50,and the virus amplification efficiency was the highest.The virus content could reach 10^(6.53) TCID_(50)/mL at 108 h.

关 键 词：VERO细胞 悬浮培养 鸡传染性支气管炎病毒 微载体 感染复数 

分 类 号：S855.3[农业科学—临床兽医学]