新疆棉花蚜虫-寄生蜂食物网结构的分子检测体系  

A molecular detection approach for assessing cotton aphid-primary parasitoid-hyperparasitoid food webs in Xinjiang,China

作　　者：李金花[1,2] 李海强 杨帆[1,4] 吴月坤[1] 张建萍 陆宴辉 Li Jinhua;Li Haiqiang;Yang Fan;Wu Yuekun;Zhang Jianping;Lu Yanhui(State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China;Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests,Ministry of Education,College of Plant Protection,Hainan University,Haikou 570228,Hainan Province,China;Institute of Plant Protection,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,Xinjiang Uygur Autonomous Region,China;Beijing Key Laboratory of Environmental Friendly Management on Pests of North China Fruits,Institute of Plant and Environment Protection,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China;Key Laboratory of Oasis Agricultural Pest Management and Plant Protection Resources Utilization,College of Agriculture,Shihezi University,Shihezi 832003,Xinjiang Uygur Autonomous Region,China)

机构地区：[1]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193 [2]海南大学植物保护学院,热带农林生物灾害绿色防控教育部重点实验室,海口570228 [3]新疆农业科学院植物保护研究所,乌鲁木齐830091 [4]北京市农业科学院植物保护环境保护研究所,北方果树病虫害绿色防控北京市重点实验室,北京100097 [5]石河子大学农学院,新疆绿洲农业病虫害治理与植保资源利用重点实验室,石河子832003

出　　处：《植物保护学报》2021年第5期970-979,共10页Journal of Plant Protection

基　　金：NSFC-新疆联合基金重点支持项目(U2003212);财政部和农业农村部国家现代农业产业技术体系(CARS-15-19)。

摘　　要：为明确新疆棉花蚜虫-寄生蜂的食物网结构,通过设计新疆棉花蚜虫和寄生蜂的特异性引物,建立并优化可以在物种水平上开展棉花蚜虫-寄生蜂食物网结构分析的分子检测方法,该方法包括3个多重PCR检测体系(cMP1、cPriMP2和cHypMP3)和1个单一PCR(cSP1)检测体系,并应用建立的方法对采自库尔勒、阿克苏、昌吉的2383头僵蚜样品进行分子检测。结果表明,4个体系的灵敏度均较高,其中蚜虫cMP1体系的检出限为500 DNA拷贝数,初级寄生蜂cPriMP2体系的检出限为5000 DNA拷贝数,重寄生蜂cHypMP3和cSP1体系的DNA检出限分别为500拷贝数和100拷贝数。该方法能够快速且准确地鉴定4种棉花蚜虫、4种初级寄生蜂和7种重寄生蜂,特异性良好。使用该方法对供试僵蚜样品进行检测并成功绘制出新疆棉花蚜虫-初级寄生蜂-重寄生蜂食物网,定量分析了不同初级寄生蜂对蚜虫的寄生作用及重寄生蜂与初级寄生蜂之间形成的食物网络关系。表明所建立的分子检测方法可以用于评估不同种类寄生蜂在新疆棉花蚜虫种群控制中的生态功能,解析物种间的食物网关系。In order to clarify the aphid-parasitoid food web structure in Xinjiang cotton fields,a molecular approach was developed and optimized based on the species-specific primers designed for cotton aphids and their parasitoids,which can be used for the aphid-parasitoid food web structure analysis at species-specific level.This molecular approach included three multiplex PCRs(cMP1,cPriMP2 and cHypMP3)and one singleplex PCR(cSP1).A total of 2383 mummy samples,collected from different areas of Xinjiang,including Korla,Changji and Aksu,were detected by this approach.The results showed that,in the sensitivity detections,the detection limits were 500,5000,500 and 100 DNA copies for aphid system cMP1,primary parasitoid system cPriMP2,hyperparasitoid systems cHypMP3 and cSP1,respectively.This molecular approach could be used for rapid and accurate identification of four aphid,four parasitoid and seven hyperparasitoid species in Xinjiang cotton fields.Based on the results of mummy samples,a tri-trophic aphid-primary parasitoid-hyperparasitoid food web was sketched,and the parasitism effect of each primary parasitoid and the interactions between different primary parasitoids and hyperparasitoids were analyzed quantitatively.The results indicated that the approach provided in the present study could be used for evaluating the ecological functions of various parasitoids in aphid population suppression and analyzing the food web interactions in Xinjiang.

关 键 词：蚜虫-初级寄生蜂-重寄生蜂 食物网 物种特异性引物 分子检测 多重PCR 

分 类 号：S476.3[农业科学—农业昆虫与害虫防治]