Nickel-Carnosine complex:A new carrier for enzymes immobilization by affinity adsorption  

作　　者：Junyang Xu Yanjun Jiang Liya Zhou Li Ma Zhihong Huang Jiafu Shi Jing Gao Ying He 

机构地区：[1]School of Chemical Engineering and Technology,Hebei University of Technology,Tianjin 300130,China [2]Tianjin Key Lab of Biomass/Wastes Utilization,School of Environmental Science&Engineering,Tianjin University,Tianjin 300072,China [3]National-Local Joint Engineering Laboratory for Energy Conservation of Chemical Process Integration and Resources Utilization,Hebei University of Technology,Tianjin 300130,China

出　　处：《Chinese Journal of Chemical Engineering》2021年第10期237-246,共10页中国化学工程学报（英文版）

摘　　要：Immobilization is an effective method to promote the application of enzyme industry for improving the stability and realizing recovery of enzyme.To some extent,the performance of immobilized enzyme depends on the choice of carrier material.Therefore,the development of new carrier materials has been one of the key issues concerned by enzyme immobilization researchers.In this work,a novel organic–inorganic hybrid material,nickel-carnosine complex(NiCar),was synthesized for the first time by solvothermal method.The obtained NiCar exhibits spherical morphology,hierarchical porosity and abundant unsaturated coordination nickel ions,which provide excellent anchoring sites for the immobilization of proteins.His-tagged organophosphate-degrading enzyme(Opd A)and x-transaminase(ω-TA)were used as model enzymes to evaluate the performance of NiCar as a carrier.By a simple adsorption process,the enzyme molecules can be fixed on the particles of NiCar,and the stability and reusability are significantly improved.The analysis of protein adsorption on NiCar verified that the affinity adsorption between the imidazole functional group on the protein and the unsaturated coordination nickel ions on NiCar was the main force in the immobilization process,which provided an idea way for the development of new enzyme immobilization carriers.

关 键 词：Nickel carnosine complex Organic-inorganic hybrid materials CARRIER ENZYMES IMMOBILIZATION 

分 类 号：TQ426.97[化学工程] Q814.2[生物学—生物工程]