组织工程化胆管研究进展  

Research progress of tissue-engineered bile duct

作　　者：冯世明 胡继仲 刘威 李立[1] FENG Shiming;HU Jizhong;LIU Wei;LI Li(Department of Hepatobiliary Surgery,Ganmei Hospital Affiliated to Kunming Medical University,Kunming 650000,P.R.China)

机构地区：[1]昆明医科大学附属甘美医院肝胆外科,昆明650000

出　　处：《中国普外基础与临床杂志》2021年第11期1504-1509,共6页Chinese Journal of Bases and Clinics In General Surgery

基　　金：国家自然科学基金项目(项目编号:81560089)。

摘　　要：目的总结近年来组织工程化胆管的研究进展。方法通过收集近年来组织工程化胆管领域中与体外测试平台、支架材料、种子细胞的获取方式及种子细胞与材料复合后的体内修复效果相关的文献,对组织工程化胆管的基础和临床应用研究情况作一综述。结果组织工程化胆管的发展极为迅速,在体外测试平台、支架材料、种子细胞、动物模型修复效果方面已经取得了长足的进展,但是在临床上的使用还有待进一步研究。体外胆管平台包括3D打印、生物模拟等;支架材料方面除了各种人工材料的进步外又引入了脱细胞基质;种子细胞的选择包括胆管来源的干细胞、人骨髓间充质干细胞(hMSCs)、肝卵圆细胞(HOC)、多能干细胞(PSCs)等各种干细胞的诱导分化;动物模型方面,组织工程化胆管在猪、狗等动物模型上也取得了良好效果。结论组织工程化胆管的发展会使肝外胆道疾病的体外基础研究更加便利,也给临床上肝外胆道损伤的治疗带来了新的选择。Objective To summarize the research progress of tissue engineered bile duct in recent years.Methods This paper summarized recently-published papers related to tissue-engineered bile duct on in vitro test platform,scaffold materials,acquisition methods of seed cells,and in vivo repair effectiveness after the fusion of seed cells and materials,in an attempt to review the basic and clinical application studies of tissue-engineered bile duct.Results Tissue-engineered bile duct had been developing rapidly.At present,great progress had been made in the fields of in vitro test platform,scaffold materials,seed cells,and repair effectiveness in animal models.However,further study was still needed in terms of its clinical application.The external bile duct platform included 3D printing and biological simulation;in the aspect of scaffold material,apart from the progress of various artificial materials,acellular matrix was introduced;the selection of seed cells included the induction and differentiation of bile duct-derived stem cells,human bone marrow mesenchymal stem cells(hMSCs),hepatic oval cell(HOC),pluripotent stem cells(PSCs),and other stem cells;animal models of tissue-engineered bile ducts had also achieved good results in animals such as pigs and dogs.Conclusion The development of tissue-engineered bile duct will promote the progress of fundamental in vitro studies on extrahepatic biliary tract diseases,thus introducing new options to the clinical treatment of extrahepatic biliary tract injuries.

关 键 词：组织工程化胆管 体外平台 支架材料 种子细胞 肝外胆道损伤 

分 类 号：R318.14[医药卫生—生物医学工程]