中心切割反相/反相二维液相色谱-串联质谱法测定烟叶潜香类糖苷  

Determination of Three Glycosides in Tobacco Using Heart-Cutting Rp/Rp Two Dimensional Liquid Chromatography-Tandem Mass Spectrometry

作　　者：陈敏[1] 董惠忠 沈世豪[1] 王天南 汪阳忠 吴达[1] 刘百战[1] CHEN Min;DONG Hui-zhong;SHEN Shi-hao;WANG Tian-nan;WANG Yang-zhong;WU Da;LIU Bai-zhan(Technological Center,Shanghai Tobacco Group Co.,Ltd.,Shanghai 200082,China)

机构地区：[1]上海烟草集团有限责任公司技术中心,上海200082

出　　处：《质谱学报》2021年第6期1175-1182,共8页Journal of Chinese Mass Spectrometry Society

摘　　要：为解决烟叶潜香类糖苷含量低、基质干扰严重、难以准确测定的问题,本研究建立了中心切割反相/反相二维液相色谱-串联质谱法定量检测烟叶中3种潜香类糖苷。样品前处理简单,仅需提取后直接上样,方法的日内和日间重复性相对标准偏差(RSD)分别为1.0%~3.2%和2.8%~8.4%,可以满足大量烟叶样品的日常检测需求。对烤烟、白肋烟、香料烟、晒烟等不同类型烟叶进行测定,结果表明,不同类型烟叶还原糖含量与潜香类糖苷呈正相关。对不同香型、不同产地的157个烤烟样品的糖苷含量进行分析,结果表明,烤烟糖苷含量不仅受还原糖的影响,还可能受类胡萝卜素、成熟期温度等多种因素的影响。A method for glycosides detection in tobacco leaves by heart-cutting Rp/Rp two-dimensional liquid chromatography-tandem mass spectrometry was established.Three glycosides were determined,including 3-oxo-7,8-dihydro-α-ionol-β-D-glucopyranoside,3-oxo-α-ionol-β-D-glucopyranoside and 3-hydroxy-β-dihydro-damascone-β-D-glucopyranoside.Only extraction and filtration were required in sample pretreatment.The RSDs of intra-day and inter-day were in the range of 1.0%-3.2%and 2.8%-8.4%,respectively,which showed good reproducibility.Since sample pretreatment was very simple,the method was quite suitable for daily detection.Different types of tobacco were determined,including Virginian-type tobacco,Burley tobacco,oriental tobacco and sun-cured tobacco.The results revealed that glycosides in flue-cured and fresh Virginian-type tobacco were significantly higher than other types,which agreed well with previous reported articles.157 kinds of Virginian-type tobacco samples from different regions were monitored and investigated.Among the factors,which influenced contents of glucosides in Virginian-type tobacco,sugar was no longer the major one,catabolite of carotenoids(C13-isoprenol glucosides)and temperature were also considered an important factor in our study.

关 键 词：中心切割反相/反相二维液相色谱-串联质谱 烟叶 糖苷 烤烟 

分 类 号：O657.63[理学—分析化学]