亚高温环境下番茄对细菌性斑点病病原菌的抗性变化及其机制研究  

Effects and The Underlying Mechanism of Sub-high Temperature on Plant Resistance to Pseudomonas syringae in Tomato

作　　者：王安然 史军营 胡璋健 王娇 喻景权[1] 师恺 WANG Anran;SHI Junying;HU Zhangjian;WANG Jiao;YU Jingquan;SHI Kai(College of Agriculture and Bio-technology,Zhejiang University,Hangzhou 310058,Zhejiang,China)

机构地区：[1]浙江大学农业与生物技术学院,浙江杭州310058

出　　处：《中国蔬菜》2021年第11期37-44,共8页China Vegetables

基　　金：国家自然科学优秀青年基金项目(31822046);浙江省自然科学基金杰青项目(LR19C150001)。

摘　　要：在全球气候变暖的大背景下,园艺作物在设施及露地生产中病虫害高发频发,严重威胁园艺产品的产量、品质和安全,而亚高温环境如何影响园艺作物的抗病性尚不清楚。细菌性斑点病是由丁香假单胞菌番茄致病变种(Pseudomonas syringae pv.tomato,Pst)引起的在我国番茄生产中广泛发生的重要病害。本试验以含抗Pst基因Pto的高抗番茄LA3472及其背景基因型高感番茄Moneymaker为试验材料,探究亚高温环境下番茄的模式触发的免疫(PAMPs-triggered immunity,PTI)和效应蛋白触发的免疫(effector-triggered immunity,ETI)两种不同抗病途径对Pst DC3000的响应变化。结果表明:与24 ℃常温相比,30 ℃亚高温对体外培养的Pst DC3000菌株的增殖速度无明显影响,但Moneymaker和LA3472在接种Pst DC3000后,细菌性斑点病害的发生均显著加重。亚高温下,2份番茄材料的水杨酸(SA)积累量在接种病原菌后均比常温下接种显著减少,受Pst DC3000诱导的水杨酸合成基因PALs和信号转导基因NPR1的转录表达也被显著抑制。由此可见,亚高温对番茄对细菌性斑点病的不同抗性途径PTI和ETI均有抑制作用,推测其和SA抗性路径被抑制有关。本试验对于理解温度升高背景下蔬菜作物病害发生及加强病害防控具有理论和现实意义。Global warming affects the interactions between horticultural crops and pathogens in both greenhouse and open field cultivation,posing a severe threat to the yield,quality,and safety of horticultural products.However,the effects of sub-high temperatures on the defense responses of horticultural crops remain unclear.Pseudomonas syringae pv.tomato(Pst),the causal agent of bacterial speck disease,widely infects tomato cultivation in China.The high-resistant tomato‘LA3472’with Pto gene resistant to Pst,and its background genotype,high-sensitive tomato‘Moneymaker’,were used as test materials,to investigate two different resistance pathways of tomato against Pst DC3000,PAMPs-triggered immunity(PTI)and effector-triggered immunity(ETI),at sub-high temperature.Results showed that sub-high temperature had no significant effect on the in vitro growth of Pst model strain Pst DC3000,but significantly increased the occurrence of speck disease in both‘Moneymaker’and‘LA3472’.Moreover,sub-high temperature remarkably suppressed the Pst DC3000-induced salicylic acid(SA)accumulation and transcript abundance of SA-biosynthetic gene PALs and SA signal-related gene NPR1 in both tomato genotypes.These results suggest that sub-high temperature has inhibitory effects on both PTI and ETI,and it is inferred to be related to the inhibition of SA resistance pathway.This research has theoretical and practical significance for understanding the occurrence of horticultural crops diseases and strengthening disease prevention under climate warming conditions.

关 键 词：番茄 亚高温 丁香假单胞菌番茄致病变种 水杨酸 抗病性 

分 类 号：S436.412.1[农业科学—农业昆虫与害虫防治]