丹酚酸B干预去卵巢骨质疏松模型大鼠的生物学变化及机制  

Biological changes and mechanism of salvianolic acid B in ovariectomized osteoporosis rat models

作　　者：王东 丁海[1] 常文举[1] 王金子 Wang Dong;Ding Hai;Chang Wenju;Wang Jinzi(Department of Orthopedics,First Affiliated Hospital of Bengbu Medical College,Anhui Tissue Transplantation Laboratory,Bengbu 233000,Anhui Province,China)

机构地区：[1]蚌埠医学院第一附属医院骨科,安徽省组织移植实验室,安徽省蚌埠市233000

出　　处：《中国组织工程研究》2022年第12期1927-1930,共4页Chinese Journal of Tissue Engineering Research

基　　金：蚌埠医学院自然科学重点项目(2020byzd096),项目负责人:常文举。

摘　　要：背景:丹酚酸B抗骨质疏松的机制不同于传统抗骨吸收药物,呈多靶点的药理效应,目前尚未完全阐明。目的:研究丹酚酸B对骨质疏松大鼠股骨与腰椎骨密度的影响,探讨其抗骨质疏松的作用机制。方法:6月龄SPF级SD大鼠36只,随机分成3组:假手术组、骨质疏松组、丹酚酸B组。骨质疏松组和丹酚酸B组行大鼠双侧卵巢摘除术,复制骨质疏松症模型;假手术组保留完整卵巢,取出部分脂肪组织。术后4周,丹酚酸B组给予50%丹酚酸B 25 mg/kg,每周灌胃给药6 d,持续12周,其余2组给予等量生理盐水,时间相同。术后16周,麻醉后处死全部大鼠,采用双能X射线吸收仪扫描大鼠右后股骨和腰椎骨密度,酶联免疫吸附法检测血清骨保护素、核因子κB受体活化因子配体水平。实验方案经蚌埠医学院动物管理委员会审批,批号:[2021]第255号。结果与结论:(1)骨质疏松组大鼠股骨骨密度比假手术组低(P<0.05),丹酚酸B组大鼠股骨骨密度比骨质疏松组高,但差异无显著性意义(P>0.05);骨质疏松组大鼠腰椎骨密度较假手术组差异无显著性意义(P>0.05),丹酚酸B组腰椎骨密度较骨质疏松组增高,但差异无显著性意义(P>0.05);(2)骨质疏松组大鼠血清骨保护素水平明显低于假手术组(P<0.05),丹酚酸B组大鼠血清骨保护素水平比骨质疏松组高,但差异无显著性意义(P>0.05);骨质疏松组大鼠血清核因子κB受体活化因子配体水平明显高于假手术组(P<0.05),丹酚酸B组大鼠血清核因子κB受体活化因子配体比骨质疏松组低,但差异无显著性意义(P>0.05);(3)结果说明,绝经后骨质疏松症早期大鼠股骨骨密度减少较为显著,腰椎骨密度无显著变化;目前尚不能认为短期单独应用丹酚酸B可以有效治疗骨质疏松症。BACKGROUND:The anti-osteoporotic mechanism of salvianolic acid B is different from traditional anti-bone resorption drugs.It has a multi-target pharmacological effect,which has not yet been fully elucidated.OBJECTIVE:To study the effect of salvianolic acid B on bone density of femur and lumbar vertebra in rats with osteoporosis,and to explore its anti-osteoporotic mechanism.METHODS:Totally 36 SPF SD rats aged 6 months were randomly divided into three groups:sham operation group,osteoporosis group,and salvianolic acid B group.Bilateral ovaries were removed and the osteoporosis model was replicated in the osteoporosis group and salvianolic acid B group.In the sham operation group,intact ovaries were kept and part of adipose tissue was removed.At 4 weeks after surgery,50%salvianolic acid B 25 mg/kg was given by gavage for 6 days per week for 12 consecutive weeks in the salvianolic acid B group.In the other two groups,the same amount of normal saline was given for the same time.At 16 weeks postoperatively,all the rats were sacrificed after anesthesia.The bone density of the right posterior femur and lumbar vertebra was scanned by dual-energy X-ray absorptiometry.The levels of osteoprotegerin and receptor activator of nuclear factor-kB ligand in serum were detected by enzyme-linked immunosorbent assay.The protocols were approved by the Animal Management Committee of Bengbu Medical College(approval No.[2021]255).RESULTS AND CONCLUSION:(1)The bone mineral density of femur in the osteoporosis group was lower than that in the sham group(P<0.05).The femoral bone mineral density of salvianolic acid B group was significantly higher than that of osteoporosis group(P>0.05).The bone mineral density of lumbar vertebrae in osteoporosis group was not significantly different from that in sham operation group(P>0.05).The lumbar vertebrae bone density of salvianic acid B group was higher than that of osteoporosis group,but the difference was not significant(P>0.05).(2)Osteoprotegerin level of osteoporosis group was lower than that of

关 键 词：大鼠 丹酚酸B 骨质疏松 骨保护素 RANKL 

分 类 号：R446[医药卫生—诊断学] R496[医药卫生—临床医学]