碱热处理联合紫外线光催化纯钛对成骨细胞活性的影响  

Effects of alkali-and-heat treatment combined with ultraviolet photofunctionalization pure titanium on osteoblast activity

作　　者：郑锐 孙勇 Zheng Rui;Sun Yong(Department of Implantology,Mianyang Stomatological Hospital,Mianyang 621000,Sichuan Province,China;School of Stomatology,Southwest Medical University,Luzhou 646000,Sichuan Province,China)

机构地区：[1]绵阳口腔医院种植科,四川省绵阳市621000 [2]西南医科大学口腔医学院,四川省泸州市646000

出　　处：《中国组织工程研究》2022年第34期5486-5491,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:钛表面经过碱热处理后会形成一层钛酸钠凝胶层促进类骨磷灰石的形成,从而与活骨发生结合;钛表面经过紫外线光催化后会形成亲水性表面,增加表面自由能,促进在体液中吸附蛋白质,进一步促进细胞与钛表面的黏附和反应。目的:探索碱热处理联合紫外线光催化纯钛表面的的理化性质,以及其对小鼠胚胎成骨细胞前体细胞MC3T3-E1黏附、增殖及分化的影响。方法:将TA2纯钛片分3组,A组进行酸蚀处理,B组进行酸蚀+碱热处理,C组进行酸蚀+碱热+紫外线光催化处理,表征3组钛片的微观形貌、元素组成及静态接触角。将对数生长期的MC3T3-E1细胞接种至3组钛片表面,采用CCK-8法检测细胞增殖情况,扫描电镜下观察细胞黏附情况;成骨诱导培养后,检测细胞的碱性磷酸酶活性、Ⅰ型胶原分泌与矿化结节形成情况。结果与结论:(1)扫描电镜下可见,A组钛片表面粗糙不平呈蜂窝状,分布着较为均匀的孔隙;B组钛片表面疏松分布着纳米级孔隙与"脑回路"状的沟壑,沟壑孔隙相互交联;C组钛片具有纳米结构,孔隙的分布、大小与B组无明显区别;(2)3组钛片表面均有C、O、Ti、P元素,B、C组钛片表面还检测出Ca元素,A组表面几乎无Ca元素;A组钛片表面静态接触角最小,C组最大;(3)接种后24 h扫描电镜下可见,各组钛片表面的细胞较多,A、B组细胞呈长梭形、三角形或扁平形,C组细胞直径增大且具有数条伪足,呈扁平的多边形在钛片表面铺展成一薄层,相互连接,无法分清细胞边界;(4)C组钛片表面的细胞增殖快于A、B组,碱性磷酸酶活性、Ⅰ型胶原分泌与矿化结节数量多于A、B组;(5)结果表明,碱热处理联合紫外线光催化能有效改善纯钛表面的理化性质,促进成骨细胞的生物活性。BACKGROUND:After alkali-and-heat treatment on the titanium surface,a layer of sodium titanate gel will be formed to promote the formation of bone-like apatite,thereby combining with living bone.The titanium surface will form a hydrophilic surface after ultraviolet photofunctionalization,which can increase the surface free energy,promote the adsorption of protein in body fluid,and further promote the adhesion and reaction between cells and titanium surface.OBJECTIVE:To explore the physical and chemical properties of alkali-and-heat treatment combined with ultraviolet photofunctionalization pure titanium surface,and its effects on adhesion,proliferation and differentiation of MC3T3-E1 cells.METHODS:TA2 pure titanium discs were randomly divided into three groups.The group A received acid etching treatment.The group B received acid etching+alkali-and-heat treatment.The group C received acid etching+alkali-and-heat+ultraviolet photofunctionalization treatment.The microscopic morphology,element composition,and static contact angle were characterized in the three groups of titanium discs.MC3T3-E1 cells in logarithmic growth phase were inoculated on the surface of three groups of titanium discs.CCK-8 assay was used to detect cell proliferation.Cell adhesion was observed under scanning electron microscope.After osteogenic induction and culture,the alkaline phosphatase activity,the secretion of type I collagen,and the formation of mineralized nodules were detected.RESULTS AND CONCLUSION:(1)Under scanning electron microscope,the surface of group A was rough and honeycomb shaped,with relatively uniform pore.The surfaces of group B were distributed with loosely nano-level pores and brain-circuit like gullies.The gullies and pores were cross-linked with each other.The surfaces of group C also had nanoporous network structure,and the distribution and size of gullies and pores were not significantly different from that of group B.(2)C,O,Ti,and P elements were both found on the surfaces of the three groups,and Ca element was also

关 键 词：种植体 钛片 紫外线 光催化 碱热处理 成骨细胞 表面处理 

分 类 号：R459.9[医药卫生—治疗学] R318.08[医药卫生—临床医学]