鸢尾素对呼吸机相关性肺损伤大鼠细胞焦亡的影响  

Effect of irisin on pyroptosis in rats with ventilator-induced lung injury

作　　者：李秋杰[1] 张鲁阳 马福国[1] 孙晓鹏[1] 张高峰[1] 孙立新[1] 王明山[1] Li Qiujie;Zhang Luyang;Ma Fuguo;Sun Xiaopeng;Zhang Gaofeng;Sun Lixin;Wang Mingshan(Department of Anesthesiology,Qingdao Municipal Hospital,Qingdao 266071,China)

机构地区：[1]青岛市市立医院东院麻醉科,266071

出　　处：《中华麻醉学杂志》2021年第8期970-974,共5页Chinese Journal of Anesthesiology

摘　　要：目的探讨鸢尾素对呼吸机相关性肺损伤大鼠细胞焦亡的影响。方法健康清洁级雄性SD大鼠36只,体重200~250 g,6~8周龄,采用随机数字表法分为3组(n=12):对照组(C组)、呼吸机相关性肺损伤组(V组)和呼吸机相关性肺损伤+鸢尾素组(V+I组)。V+I组机械通气前尾静脉注射鸢尾素1μg/kg。机械通气(潮气量40 ml/kg,通气频率60次/min,吸呼比设为1∶2,PEEP 0,FiO221%)4 h后采集股动脉血样,进行动脉血气分析,记录PaO2,计算氧合指数(OI);收集支气管肺泡灌洗液(BALF),测定总蛋白浓度,采用ELISA法测定BALF和血清IL-1β、IL-18浓度。取肺组织,HE染色后行肺损伤评分,测定肺组织湿重/干重(W/D)比值;分别采用Western blot法和RT-PCR法检测焦亡相关蛋白消皮素D-N端(GSDMD-N)、caspase-1及其mRNA的表达水平。结果与C组比较,V组肺损伤评分和W/D比值升高,PaO2和OI降低,BALF总蛋白浓度、BALF和血清IL-1β、IL-18浓度升高,肺组织caspase-1、GSDMD-N及其mRNA表达上调(P<0.01);与V组比较,V+I组肺损伤评分和W/D比值降低,PaO2和OI升高,BALF总蛋白浓度、BALF和血清IL-1β、IL-18浓度降低,肺组织caspase-1、GSDMD-N及其mRNA表达下调(P<0.01)。结论鸢尾素减轻大鼠呼吸机相关性肺损伤的机制可能与抑制细胞焦亡有关。Objective To investigate the effect of irisin on pyroptosis in rats with ventilator-induced lung injury.Methods Thirty-six healthy clean-grade male Sprague-Dawley rats,weighing 200-250 g,aged 6-8 weeks,were divided into 3 groups(n=12 each)using a random number table method:control group(group C),ventilator-induced lung injury group(group V)and ventilator-induced lung injury plus irisin group(group V+I).In group V+I,irisin 1μg/kg was injected via the tail vein before mechanical ventilation.The animals were mechanically ventilated(tidal volume of 40 ml/kg,respiratory rate 60 breaths/min,inspiratory/expiratory ratio 1∶2,positive end expiratory pressure 0 and inspired oxygen fraction ratio 21%.Blood samples were then taken from the femoral artery for blood gas analysis,and PaO2 was recorded.Bronchoalveolar lavage fluid(BALF)was collected,the total protein concentrations in BALF were measured,and the concentrations of BALF and serum interleukin-1β(IL-1β)and IL-18 were measure by enzyme-linked immunosorbent assay.The lung tissues were obtained for determination of the pathological changes after HE staining which were scored,wet to dry weight(W/D)ratio,expression of pyroptosis-related proteins N-terminal gasdermin D(GSDMD-N)and caspase-1 protein and mRNA(by Western blot or using real-time polymerase chain reaction).Results Compared with group C,the lung injury score and W/D ratio were significantly increased,PaO2 and OI were decreased,the total protein concentrations in BALF,concentrations of IL-1βand IL-18 in BALF and serum were increased,and the expression of caspase-1 and GSDMD-N protein and mRNA was up-regulated in group V(P<0.01).Compared with group V,the lung injury score and W/D ratio were significantly decreased,PaO2 and OI were increased,the total protein concentrations in BALF,concentrations of serum IL-1βand IL-18 in BALF and serum were decreased,and the expression of caspase-1 and GSDMD-N protein and mRNA was down-regulated in group V+I(P<0.01).Conclusion The mechanism by which irisin reduces ventilat

关 键 词：纤连蛋白类 细胞焦亡 呼吸机相关性肺损伤 

分 类 号：R614[医药卫生—麻醉学]