UPLC-QTOF-MS法探讨温肾清毒方对大鼠生殖器官中依非韦伦浓度的影响  

Effect of Wenshen Qingdu Recipe on Efavirenz Concentration in Reproductive Organs of Rats by UPLC-QTOF-MS Method

作　　者：陆珍珍[1] 王绍龙[1] 钟鹏程 赵江月 阮志 李庆波 符林春[2] LU Zhen-zhen;WANG Shao-long;ZHONG Peng-cheng;ZHAO Jiang-yue;RUAN Zhi;LI Qing-bo;FU Lin-chun(Guangxi Center for Disease Control and Prevention,Nanning 530028;Science and Technology Innovation Center,Guangzhou University of Chinese Medicine,Guangzhou 530405)

机构地区：[1]广西疾病预防控制中心,南宁530028 [2]广州中医药大学科技创新中心,广州530405

出　　处：《中国中西医结合杂志》2021年第11期1336-1340,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：广西艾滋病防治研究重点实验室开放课题项目(No.gklapt201702);广西自然科学基金项目(No.2020GXNSFAA238044)。

摘　　要：目的探索温肾清毒方"艾可清"对大鼠生殖器官中依非韦伦浓度(EFV)的影响。方法24只雄性SD大鼠随机分为4组,EFV+高剂量艾可清组、EFV+低剂量艾可清组、EFV组及空白组,每组6只。大鼠灌胃给药7天后处死;腹腔静脉采血,取上清液;取前列腺、睾丸、附睾、膀胱组织,按组织和4℃双蒸水1:2比例配置匀浆液。超高效液相色谱四极杆飞行时间质谱分析(UHPLC-QTOF/MS)检测血清和匀浆中EFV响应浓度。结果空白组无离子响应。EFV组、EFV+高剂量组、EFV+低剂量组各组内血浆和组织中离子响应浓度比较差异无统计学意义(均P>0.05);3个组的血浆的EFV浓度比较差异无统计学意义(P>0.05),但是EFV+高剂量艾可清组及EFV+低剂量艾可清组组织中EFV浓度高于EFV组(P=0.018)。各组组织和血浆中EFV的分布如下:EFV+低剂量组>EFV+高剂量组>EFV组。结论艾可清能够提高大鼠生殖器官组织中EFV浓度。Objective To explore effect of Wenshen Qingdu Recipe"Aikeqing"(AKQ)on the concentration of efavirene(EFV)in reproductive organs of rats.Methods Twenty-four male sprague Dawley(SD)rats were divided into 4 groups,i.e.,the EFV+high dose AKQ group,EFV+low dose AKQ group,EFV group and control group,6 in each group.The rats were sacrificed after 7 days of intragastric administration,and the blood was collected from abdominal vein and supernatant was abtained.The organs(prostate,testis,epididymis and bladder)were mashed and prepared as homogenate according to the proportion of tissue and 4℃double distilled water in a ratio of 1:2.The concentration of EFV in serum and homogenate was detected with ultra-performance liquid chromatography–quadrupole time-of-flight mass spectrometry(UPLC–QTOF-MS).Results There was no ionic response in the control group.There was no significant difference ionic response concentrations in plasma and tissue in EFV group,EFV+high AKQ dose group and EFV+low dose AKQ group,Similarly,there was no significant difference in plasma EFV concentration among the three groups(P>0.05).Whereas,compared with the EVF group,EFV concentration in tissues were increased in EFV+high dose AKQ group and EFV+low AKQ dose group(P=0.018).It showed higher concentration for EFV+low dose AKQ group versus EFV+high dose AKQ group,following by EVF group.Conclusion AKQ could increase the concentration of EFV in the reproductive organs of rats.

关 键 词：人类免疫缺陷病毒 艾可清 依非韦伦 浓度 生殖系统 大鼠 中西医结合 

分 类 号：R285.5[医药卫生—中药学]