高分子混合刷吸附/脱附蛋白质的模型化研究  

Modeling study of adsorption/desorption of proteins by polymer mixed brush

作　　者：刘春杰 赵新军 高志福[3] 蒋中英 Liu Chun-Jie;Zhao Xin-Jun;Gao Zhi-Fu;Jiang Zhong-Ying(Xinjiang Laboratory of Phase Transitions and Microstructures of Condensed Matter Physics,Yi Li Normal University,Yining 835000,China;Laboratory of Micro-Nano Electro Biosensors and Bionic Devices,Yi Li Normal University,Yining 835000,China;Xinjiang Astronomical Observatory,Chinese Academy of Sciences,Urumqi 830011,China)

机构地区：[1]伊犁师范大学,新疆凝聚态相变与微结构实验室,伊宁835000 [2]伊犁师范大学,微纳电传感器技术与仿生器械实验室,伊宁835000 [3]中国科学院,新疆乌鲁木齐天文台,乌鲁木齐830011

出　　处：《物理学报》2021年第22期205-214,共10页Acta Physica Sinica

基　　金：国家自然科学基金项目(批准号:22163011)资助课题;伊犁师范大学博士科研启动基金资助(批准号:2020YSBS008);新疆自然科学基金联合基金项目(批准号:2019D01C333)资助的课题.

摘　　要：本文基于扩散动力学,建立了一种新的理论模型研究高分子混合刷在蛋白质吸附/脱附过程中的动力学特性.理论模型考虑高分子混合刷中一种高分子链(P-高分子链)对蛋白质的吸附,另一种高分子链(N-高分子链)对蛋白质的脱附,以及吸附/脱附的时滞性.通过选取模型中各参数值,获得了具有不同化学、物理性质的高分子混合刷对蛋白质的部分吸附/脱附、完全吸附/脱附,以及周期性吸附/脱附的动力学特性.研究发现,由于外加交变电场的作用,高分子混合刷对蛋白质吸附/脱附过程呈现出周期性循环的动力学特性,并且平均吸附、脱附量增加.本文理论结果符合实验观测.可以预言,外加交变电场可实现高分子混合刷对蛋白质吸附/脱附的多次循环,为设计吸附/脱附蛋白质的高分子混合刷纳米材料提供必要的参考和新方案.Based on the diffusion dynamics,a new theoretical model is established to investigate the dynamic properties of a polymer mixed brush(PMB)in the protein adsorption/desorption process.The theoretical model considers the adsorption of proteins by one polymer chain(P-polymer chain)and the desorption of proteins by another polymer chain(N-polymer chain)in a PMB,as well as the time delay between adsorption and desorption.The dynamic properties of protein adsorption/desorption by a PMB depend on not only the chemical and physical properties of polymer chains,but also the microenvironment(density of protein in the solution and protein diffusivity)of the PMB.In order to describe the different chemical and physical properties of polymer chains and microenvironments in PMB,we take different model parameters,and obtain partial adsorption/desorption,complete adsorption/desorption and periodic adsorption/desorption of proteins by the PMB.By analyzing the process of protein adsorption/desorption in a PMB,we find that the microenvironment has an obvious influence on the adsorption and desorption of protein by the PMB.It is also shown that the adsorption of protein and the desorption of protein by the PMB have a stable and invariable periodic cycle when an alternating electric field is applied.The average adsorption capacity and the average desorption capacity increase in comparison with those when no electric field is applied.A stable alternating electric field enables the PMB to exhibit stable periodic dynamic characteristics in the dynamic process of protein adsorption and desorption.Our theoretical results are consistent with the experimental observations.Based on this,it is predicted that an external electric field can realize multiple cycles of protein adsorption and desorption by PMB,which provides necessary references and useful insights into controllable protein adsorption/desorption by the PMB in the practical applications.

关 键 词：高分子混合刷 蛋白质 吸附/脱附 动力学特性 

分 类 号：TQ317[化学工程—高聚物工业] O647.3[理学—物理化学]