Morphological and phylogenetic diversity of magnetotactic bacteria and multicellular magnetotactic prokaryotes from a mangrove ecosystem in the Sanya River,South China  

作　　者：Haitao CHEN Jinhua LI Long-Fei WU Wei-Jia ZHANG 

机构地区：[1]Beijing Key Laboratory of Biological Electromagnetism,Institute of Electrical Engineering,Chinese Academy of Sciences,Beijing 100190,China [2]France-China Joint Laboratory for Evolution and Development of Magnetotactic Multicellular Organisms,Chinese Academy of Sciences,Beijing 100029,China [3]Deep-Sea Microbial Cell Biology,Department of Deep Sea Sciences,Institute of Deep-Sea Science and Engineering,Chinese Academy of Sciences,Sanya 572000,China [4]Key Laboratory of Earth and Planetary Physics,Institute of Geology and Geophysics,Chinese Academy of Sciences,Beijing 100029,China [5]LCB,Aix Marseille University,CNRS,Marseille 13402,France

出　　处：《Journal of Oceanology and Limnology》2021年第6期2015-2026,共12页海洋湖沼学报（英文）

基　　金：Supported by the Project of Academy Locality Science and Technology Cooperation of Sanya City,China(No.2014YD02);the National Natural Science Foundation of China(No.41920104009)。

摘　　要：Magnetotactic bacteria(MTB)are morphologically and phylogenetically diverse prokaryotes commonly able to produce magnetic nanocrystals within intracellular membrane-bound organelles(i.e.,magnetosomes)and to swim along geomagnetic field lines.We studied the diversity of MTB in the samples collected from a mangrove area in the Sanya River,Hainan,South China,using microscopic and microbial phylogenetic methods.Results of microanalysis and observation in microscopy and energy dispersive X-ray spectroscopy(EDXS)reveal a highly morphological diversity of MTB including unicellular cocci,vibrios,rod-shaped bacteria,and three morphotypes of multicellular magnetotactic prokaryotes(MMPs).In addition,analysis of the 16S rRNA gene showed that these MTB were clustered into 16 operational taxonomic units affi liated to the Alpha-,Delta-,and Gamma-proteobacteria classes within the Proteobacteria phylum.Meanwhile,by using the coupled fluorescence and transmission electron microscopy analysis,rodshaped bacteria,vibrio,and cocci were phylogenetically and structurally identified at the single-cell level.This study demonstrated highly diverse MTB communities in the mangrove ecosystem and provide a new insight into the overall diversity of MTB.

关 键 词：magnetotactic bacteria diversity MAGNETOSOME phylogenetic analysis coordinated fl uorescence in-situ hybridization transmission electron microscopy(FISH-TEM) MANGROVE 

分 类 号：Q64[生物学—生物物理学]