Genome-wide evolution of MAPKs family and their expression in response to bacterial infection in seahorse Hippocampus erectus  

作　　者：Kai WANG Xin WANG Qiang ZOU Han JIANG Rongrong ZHANG Yanan TIAN Lele ZHANG Qiang LIN 

机构地区：[1]School of Agriculture,Ludong University,Yantai 264025,China [2]CAS Key Laboratory of Tropical Marine Bio-resources and Ecology,South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301,China [3]Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou),Guangzhou 511458,China [4]Yantai Branch of Shandong Technology Transfer Center,Chinese Academy of Sciences,Yantai 264003,China [5]University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Journal of Oceanology and Limnology》2021年第6期2309-2321,共13页海洋湖沼学报（英文）

基　　金：Supported by the Shandong Province Science and Technology Support Program for Outstanding Youth of Colleges and Universities(No.2020KJF007);the Shandong Province Science and Technology Research Program for Colleges and Universities(No.J18KA146);the Yantai Foundation for Development of Science and Technology(Nos.2020LJRC120,2019CXJJ040);the Weihai Foundation for Development of Science and Technology(No.2017GNS10);the Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou)(No.GML2019ZD0407);the Guangdong Basic and Applied Basic Research Foundation(No.2019A1515110199)。

摘　　要：Seahorses have evolved many unique biological traits,including a male brood pouch,the absence of caudal and pelvic fins,and the lack of spleen and gut-associated lymphatic tissue.The mitogenactivated protein kinases(MAPKs)are known to be involved in various important biological processes including growth,differentiation,immunity,and stress responses.Therefore,we hypothesized that the adaptive evolution and expression of the MAPK gene family in seahorse may differ from those of other teleost species.We identified positive selection sites in the erk2,erk5,jnk1,and p38αMAPK genes of the lined seahorse Hippocampus erectus and tiger-tailed seahorse Hippocampus comes.A novel expression profile of MAPK cascade genes was found in seahorse larvae during the first day after birth based on the RNA-seq data of H.erectus,which refl ected vital signs of immune response to its parental immune system.The expression patterns of the four positively selected MAPK genes were analyzed following the bacterial challenge of Vibrio fortis,revealing their upregulation pattern in brood pouch and other immune tissues.This study enriched our knowledge of the evolution of the H.erectus MAPK subfamilies,and could help better understanding the functional role of MAPKs in teleosts.

关 键 词：mitogen-activated protein kinases(MAPKs) Hippocampus erectus genomic structure positive selection immune regulation 

分 类 号：Q953[生物学—动物学]