基于DNA条形码的柳属植物系统发育研究  

Phylogenetic Analysis of DNA Barcoding Sequences in the Genus Salix

作　　者：任伟超[1] 徐姣[2] 孙伟[3] 刘美琦 于欣欣 王思嘉 马伟[1] REN Wei-Chao;XU Jiao;SUN Wei;LIU Mei-Qi;YU Xin-Xin;WANG Si-Jia;MA Wei(Pharmacy College,Heilongjiang University of Chinese Medicine,Harbin 150040;Jiamusi College,Heilongjiang University of Chinese Medicine,Jiamusi 154007;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700)

机构地区：[1]黑龙江中医药大学药学院,哈尔滨150040 [2]黑龙江中医药大学佳木斯学院,佳木斯154007 [3]中国中医科学院中药研究所,北京100700

出　　处：《植物研究》2021年第6期896-903,共8页Bulletin of Botanical Research

基　　金：国家中医药管理局全国中药资源普查项目(GZY-KJS-2018-004);黑龙江中医药大学优秀创新人才领军人才项目(2018RCL09)。

摘　　要：对黑龙江省柳属不同植物ITS2和psbA-trnH基因间隔区序列进行系统发育分析,探索柳属植物的遗传多样性和亲缘关系。提取柳属植物和柽柳样品基因组DNA,通过PCR扩增法,获得各样品ITS2和psbA-trnH序列并测序。通过对序列种内种间遗传距离及邻接法构建系统发育树进行分析。基于ITS2序列的DNA条形码鉴定方法能将五蕊柳、粉枝柳、筐柳、蒿柳与其他物种区分开,柳组物种仅可鉴定到组级别。在以psbA-trnH基因片段序列建立的系统发育树中,柳属各种之间的序列聚类较为混乱,并不能各自独立聚为一支。因此,ITS2序列在柳属植物分类鉴定上具有一定的优势,为柳属植物的开发利用提供参考依据。We used phylogenetic analysis to compare the ITS2 and psbA-trnH sequences of the plant of the genus Salix samples,so as to explore the genetic diversity and genetic relationship of these resources.Extract genomic DNA of the genus Salix and Tamarix chinensis samples.The ITS2 and psbA-trnH gene sequences of each samples were amplified and sequenced by PCR.The intraspecific and interspecific genetic distances and the neighbor joining(NJ)methods were used to construct phylogenetic trees.According to the phylogenetic tree of ITS2.S.pentandra,S.rorida,S.linearistipularis and S.schwerinii formed a monophyletic clade,distinguished from the same genus.Phylogenetic tree constructed by psbA-trnH sequences were unable to distinguish the genus Salix samples from different geographical sources.Therefore,ITS2 sequence was suitable for the authentication,and would provide a certain reference for the subsequent development and research of the plant of genus Salix.

关 键 词：柳属 ITS2 PSBA-TRNH DNA条形码 分子鉴定 

分 类 号：S792.12[农业科学—林木遗传育种]