PEG渗透处理水曲柳种子影响其热休眠诱导及水分状态的关联性研究  

Effects of PEG Osmotic Treatment on Thermal Dormancy Induction and Water Status of Fraxinus mandshurica Seeds

作　　者：崔程程 李明月[1] 张鹏[1] CUI Cheng-Cheng;LI Ming-Yue;ZHANG Peng(Key Laboratory of Sustainable Forest Ecosystem Management Minisrty of Education,School of Forestry,Northeast Forestry University,Harbin150040)

机构地区：[1]东北林业大学林学院,森林生态系统可持续经营教育部重点实验室,哈尔滨150040

出　　处：《植物研究》2021年第6期904-910,共7页Bulletin of Botanical Research

基　　金：国家自然科学基金项目(31870615)。

摘　　要：以解除初生休眠的水曲柳种子为材料,经过预吸水和未预吸水的两批种子,在25℃条件下,用不同浓度(0、25%、50%)的PEG处理不同时间(3天、7天、3天PEG环境+4天水环境)后,适宜条件下萌发,测定不同处理过程中种子的含水量变化以及种子萌发表现,以此来探究种子经不同浓度PEG渗透处理后的含水量变化规律及其与种子发芽能力的关系。结果表明:水曲柳种子经PEG处理后种子含水率:对照>25%PEG>50%PEG,即PEG浓度越低,种子含水率越高;其中,PEG处理后,经预吸水的种子含水率降低,而未预吸水的种子含水率逐渐升高,相同PEG浓度处理相同时间时,预吸水与未预吸水的水曲柳种子萌发率有明显差异,即PEG处理和预吸水与否均影响种子含水率变化规律和种子发芽能力;种子含水率与种子发芽率成负相关。因此,PEG渗透处理减弱水曲柳种子热休眠诱导的作用与种子水分状态有关。Taking the seeds of Fraxinus mandshurica as the material to release the initial dormancy,after two batches of seeds of pre-water absorption and non-pre-water absorption,at 25℃,with different concentrations(0,25%,50%)of PEG treatment different time(3 days,7 days,3 days PEG+4 days H2O environment)and germination under suitable conditions.The changes of seed water content and seed germination performance during different treatments were determined to explore the changes of water content of seeds treated with PEG at different concentrations and the relationship between the water content and seed germination ability.The results showed that:after PEG treatment,the seed water content of seeds was as follows:contrast>25%PEG>50%PEG,that was,the lower the PEG concentration,the higher the seed water content.Among them,after PEG treatment,the water content of seeds treated with pre-water decreased,while the water content of seeds without pre-water increased gradually.At the same PEG concentration for the same time,the germination rate of seeds treated with pre-water and non-pre-water was significantly different,that was,whether PEG treatment or pre-water treatment all affected the change law of seed water content and seed germination ability.The seed water was negatively correlated with the seed germination rate.PEG osmotic treatment attenuated the induction of thermal dormancy of F.mandshurica seeds,which was related to seed water status.

关 键 词：水曲柳 热休眠 PEG6000 种子含水率 

分 类 号：S792[农业科学—林木遗传育种]