X线片在钛合金笼植入治疗犬股骨头缺血性坏死中的作用效果评估  

Evaluation of the effect of plain X-ray film in the treatment of avascular necrosis of the femoral head in dogs with titanium alloy cage implantation

作　　者：杨娟 刘文秀[1] 师芳芳[2] 闫婧 YANG Juan;LIU Wen-xiu;SHI Fang-fang(Department of Radiology,Affiliated Hospital of Yan'an University,Yan'an Shaanxi 716000,China;School of Medicine,Yan'an University,Yan'an Shaanxi 716000,China)

机构地区：[1]延安大学附属医院放射科,陕西延安716000 [2]延安大学医学院,陕西延安716000

出　　处：《临床和实验医学杂志》2021年第22期2378-2382,共5页Journal of Clinical and Experimental Medicine

基　　金：陕西省教育厅项目(编号:15jk1813)。

摘　　要：目的评估X线片在钛合金笼植入治疗犬股骨头缺血性坏死中的作用效果。方法20只普通级健康成年雄性杂交犬,根据实验目的分为2组:对照组(n=10)通过液氮冷冻浇灌造成犬股骨头缺血性坏死模型,进行后期维护,预防伤口感染;实验组(n=10)造成股骨头缺血性坏死后进行钛合金笼植入治疗,并做好伤口护理。每天观察健康状况、伤口愈合和术后并发症。通过高级骨分析仪分析股骨头的骨矿物质密度和小梁厚度。通过使用Image-Pro Plus 6.0获得切片组织的股骨头宽度和小梁面积百分比。Masson三色染色和CD31免疫组织化学染色分析新骨形成和股骨头微血管密度。通过X线片和组织学分析对犬软骨和股骨头缺损进行评估。术后12周观察比较犬跛行状态。结果实验组较对照组骨矿物质密度和股骨头小梁厚度增大,差异均有统计学意义(P<0.05)。实验组较对照组的小梁面积百分比和小梁宽度升高,差异均有统计学意义(P<0.05)。X线片显示,对照组和实验组重建后12周时犬股骨头的术后变化。在对照组中观察到关节塌陷,实验组中股骨头的轮廓保存完好。实验组较对照组的新骨形成面积和微血管密度升高,差异均有统计学意义(P<0.05)。实验组较对照组放射学评分和组织学评分升高,差异均有统计学意义(P<0.05)。实验组较对照组犬跛行严重程度降低,差异有统计学意义(P<0.05)。结论将钛合金笼植入坏死的股骨头并没有改变股骨头的形状,通过X线片可以发现再生的海绵骨可长入笼内,重建、修复、置换坏死的骨组织,形成海绵骨网络,钛合金笼为血管内皮生长因子诱导材料的植入提供了空间。Objective To evaluate the effect of plain X-ray film in the treatment of avascular necrosis of the femoral head in dogs with titanium alloy cage implantation.Methods Twenty normal-grade healthy adult male hybrid dogs were divided into 2 groups according to the purpose of the experiment:the control group(a model of canine femoral head avascular necrosis caused by liquid nitrogen freezing,for post-maintenance to prevent Yamaguchi infection,n=10),the experimental group(after causing avascular necrosis of the femoral head)Titanium alloy cage implantation treatment,and the best wound care,n=10.Health status,wound healing and postoperative complications were observed daily.A canine model of avascular necrosis of the femoral head was induced by freezing injury with liquid nitrogen.Laboratory animal surgery procedures and feeding standards were approved in accordance with the local university's laboratory animal care and use guidelines.Analyze the bone mineral density and trabecular thickness of the femoral head by an advanced bone analyzer.The width of the femoral head and the percentage of trabecular area of the sliced tissue were obtained by using Image-Pro Plus 6.0.Masson trichrome staining and CD31 immunohistochemical staining were used to analyze new bone formation and femoral head microvessel density.The canine cartilage and femoral head defects were assessed by plain radiographs and histological analysis.The dog's lame state was observed and compared 12 weeks after the operation.Results The bone mineral density and the thickness of the femoral head trabecula in the test group were increased compared with the control group,the differences were statistically significant(P<0.05).Compared with the control group,the experimental group had higher trabecular area percentage and trabecular width(P<0.05).Representative plain radiographs show the postoperative changes of the canine femoral head at 12 weeks after reconstruction in the control group and the experimental group.Joint collapse was observed in the control group,

关 键 词：实验犬 股骨头坏死 X线片 钛合金笼 

分 类 号：R687.3[医药卫生—骨科学]