有氧运动调控自发性高血压模型大鼠心脏重塑的机制  

Regulatory mechanism of aerobic exercise on cardiac remodeling in spontaneously hypertensive rats

作　　者：朱政 付常喜[1] 马文超[1] 马刚 彭朋[2] Zhu Zheng;Fu Changxi;Ma Wenchao;Ma Gang;Peng peng(Department of Physical Education,Xuzhou University of Technology,Xuzhou 221018,Jiangsu Province,China;Department of Health Service,Logistics University of Chinese People’s Armed Police Forces,Tianjin 300309,China)

机构地区：[1]徐州工程学院体育学院,江苏省徐州市221018 [2]中国人民武装警察部队后勤学院卫生勤务系,天津市300309

出　　处：《中国组织工程研究》2022年第14期2231-2237,共7页Chinese Journal of Tissue Engineering Research

基　　金：天津市自然科学基金项目(17JCYBJC27400),项目负责人:彭朋。

摘　　要：背景:肾素-血管紧张素系统是介导高血压发生发展的主要病理生理机制。规律运动尤其是有氧运动对于诸多心血管疾病均具有防治作用,然而针对高血压的最佳运动处方仍未确定。目的:观察有氧运动对自发性高血压大鼠(SHR)心脏重塑的影响,并探讨局部和全身肾素-血管紧张素系统在其间的可能作用机制。方法:30只雄性SHR随机分为SHR安静组和SHR运动组,同时将15只Wistar-Kyoto大鼠作为正常血压对照组(WKY组)。WKY组和SHR安静组大鼠在鼠笼内安静饲养,SHR运动组进行8周中等强度跑台运动。实验后,利用无创血压仪检测尾动脉血压;超声心动仪检测心脏结构与功能;苏木精-伊红和Masson染色进行心肌组织病理学观察;荧光底物法测定血清、心脏血管紧张素转换酶和血管紧张素转换酶2活性;高效液相色谱法检测血清、心脏血管紧张素Ⅱ和血管紧张素(1-7)水平;Western bolt法检测心脏血管紧张素转换酶、血管紧张素Ⅱ1型受体(AT1R)、血管紧张素转换酶2和Mas受体蛋白表达量。研究经徐州工程学院伦理委员会审查和批准(批准号:XZUTLL 2019-003-02)。结果与结论:①心脏结构与功能:与WKY组比较,SHR安静组大鼠血压升高(P<0.05),左心室发生向心性肥大与心肌纤维化(P<0.05),心功能下降(P<0.05);与SHR安静组比较,SHR运动组血压下降(P<0.05),左心室呈现离心性肥大、心肌纤维化减轻,心功能改善(P<0.05);②局部(心脏)肾素-血管紧张素系统:与SHR安静组比较,SHR运动组心脏血管紧张素转换酶活性和蛋白表达量以及血管紧张素Ⅱ水平降低(P<0.05),血管紧张素(1-7)水平升高(P<0.05),血管紧张素转换酶2和Mas受体蛋白表达量上调(P<0.05),血管紧张素转换酶/血管紧张素转换酶2活性和蛋白表达比值、血管紧张素Ⅱ/血管紧张素(1-7)比值以及AT1R/Mas受体蛋白表达比值降低(P<0.05);③全身肾素-血管紧张素系统:各组血清血管紧�BACKGROUND:Renin-angiotensin system is the main pathophysiological mechanism that mediates the occurrence and development of hypertension.Regular exercise,especially aerobic exercise,can prevent and treat many cardiovascular diseases.However,the optimal exercise prescription for hypertension has not been determined.OBJECTIVE:To observe the effect of aerobic exercise on cardiac remodeling and to investigate the possible mechanism of local and systemic renin-angiotensin system in spontaneously hypertensive rats(SHR)METHODS:Thirty male SHR rats were randomly divided into SHR sedentary group and SHR exercise group.At the same time,15 Wistar-Kyoto rats were used as normal blood pressure control group(WKY).The animals in the WKY and SHR sedentary groups were caged and raised quietly,and the SHR exercise group performed moderate intensity treadmill exercise for 8 weeks.After the experiment,caudal artery blood pressure was measured by non-invasive sphygmomanometer.Cardiac structure and function were detected by echocardiography.Myocardial histopathology was observed by hematoxylin-eosin and Masson staining.Angiotensin converting enzyme(ACE)and ACE2 activities in serum and heart were measured by fluorescence substrate method,and angiotensin II(Ang II)and Ang(1-7)contents in serum and heart were detected by high performance liquid chromatography.The protein expression of ACE,Ang II type 1 receptor(AT1R),ACE2 and Mas receptor was detected by western blot.The study protocol was reviewed and approved by the Ethics Committee of Xuzhou University of Technology(approval No.XZUTLL 2019-003-02).RESULTS AND CONCLUSION:Cardiac structure and function:compared with the WKY group,blood pressure increased(P<0.05),left ventricular concentric hypertrophy and myocardial fibrosis occurred(P<0.05),and cardiac function decreased(P<0.05)in SHR sedentary group.Compared with the SHR sedentary group,blood pressure decreased(P<0.05),left ventricle presented eccentric hypertrophy and myocardial fibrosis alleviated(P<0.05),and cardiac function impro

关 键 词：有氧运动 高血压 肾素-血管紧张素系统 心脏重塑 心力衰竭 

分 类 号：R446[医药卫生—诊断学] R496[医药卫生—临床医学]