醋甲唑胺干预尾吊2周失重模型大鼠眼压和视网膜、脉络膜厚度的变化  

Methazolamide effects on intraocular pressure,retinal and choroidal thickness in rats with tail suspension for 2 weeks

作　　者：宫玉波[1] 张文倩[2] 郭小华 闫瑾[1] 宋飞龙 石圆圆[1] 罗灵[1] 赵军[1] 赵宏伟[1] Gong Yubo;Zhang Wenqian;Guo Xiaohua;Yan Jin;Song Feilong;Shi Yuanyuan;Luo Ling;Zhao Jun;Zhao Hongwei(Department of Ophthalmology,PLA Strategic Support Force Medical Center(formerly the 306th Hospital of the PLA),Beijing 100101,China;Department of Ophthalmology,Huantai County People’s Hospital,Zibo 256400,Shandong Province,China)

机构地区：[1]解放军战略支援部队特色医学中心(原解放军第306医院)眼科,北京市100101 [2]桓台县人民医院眼科,山东省淄博市256400

出　　处：《中国组织工程研究》2022年第14期2238-2242,共5页Chinese Journal of Tissue Engineering Research

基　　金：国家环境保护环境感官应激与健康重点实验室开放基金(19ZX84),项目负责人:宫玉波;全军医学科技青年培育项目(17QNP032),项目负责人:赵军;全军医学科技青年培育项目(16QNP035),项目负责人:赵宏伟;航天医学实验领域项目(HYZHXM01014 FB1),项目负责人:罗灵。

摘　　要：背景:失重会影响眼压及眼部结构变化;醋甲唑胺有降低眼压作用,但对失重状态下眼压及视网膜、脉络膜厚度变化的影响却鲜有报道。目的:探讨醋甲唑胺对模拟2周微重力环境下大鼠眼压及视网膜、脉络膜厚度变化的影响。方法:16只SD大鼠随机分为2组,对照组(8只)及药物组(8只),均采用-30°尾悬吊法建立模拟微重力环境大鼠模型。尾吊前(尾吊0 d)2组大鼠不给药;尾吊后对照组和药物组大鼠分别灌胃等量生理盐水或醋甲唑胺生理盐水溶液,1次/d,连续14 d。于尾吊前、尾吊1,3,7及14 d采用iCare眼压仪测量尾吊大鼠不同时段眼压变化;应用EDI SD-OCT测量尾吊大鼠不同时段视网膜、脉络膜厚度变化。实验方案经解放军战略支援部队特色医学中心动物实验伦理委员会批准。结果与结论:①眼压比较:对照组尾吊后眼压升高,尾吊后14 d眼压显著高于尾吊前(P<0.05);尾吊7 d眼压显著高于尾吊3 d(P<0.05);药物组:尾吊前与尾吊后各时间段眼压比较差异均无显著性意义(P>0.05);尾吊后药物组较对照组眼压明显下降(P<0.05);②视网膜厚度比较:2组组内不同时段视网膜厚度比较差异均无显著性意义(P>0.05);2组之间视网膜厚度比较差异无显著性意义(P>0.05);③脉络膜厚度比较:对照组尾吊后脉络膜增厚;尾吊3 d脉络膜较尾吊1 d增厚(P<0.05),但与尾吊7,14 d比较差异均无显著性意义(P>0.05);药物组尾吊后脉络膜增厚;尾吊3,7,14 d脉络膜均较尾吊1 d增厚(P<0.05);尾吊3 d与尾吊7,14 d脉络膜厚度比较差异均无显著性意义(P>0.05);尾吊后药物组较对照组脉络膜厚度明显降低(P<0.05);④结果说明,模拟2周微重力环境对大鼠眼压有明显影响;随尾吊时间延长,眼压升高,尾吊14 d眼压升高明显;醋甲唑胺有效抑制了眼压升高。模拟2周微重力环境对大鼠视网膜厚度无明显影响,对脉络膜厚度有明显影响;随尾吊时间延长,脉络膜厚度BACKGROUND:Weightlessness can affect intraocular pressure and ocular structure changes.Methazolamide can reduce intraocular pressure,but its effect on intraocular pressure,retinal and choroid thickness in weightlessness is rarely reported.OBJECTIVE:To investigate the effect of the methazolamide on intraocular pressure and retinal and choroidal thickness in rats with simulated microgravity for 2 weeks.METHODS:Sixteen healthy Sprague-Dawley rats were randomly divided into two groups:control group(n=8)and drug group(n=8).A model of simulated microgravity environment was established in each rat by-30°tail suspension method.All the rats were not treated before tail suspension,and intragastrically given normal saline and methazolamide saline solution in the two groups respectively after tail suspension,once a day,for 14 continuous days.iCare tonometer was used to measure the changes of intraocular pressure in tail-suspended rats before tail suspension and 1,3,7,and 14 days after tail suspension.EDI SD-OCT was used to measure the changes of retinal and choroidal thickness in tail-suspended rats before tail suspension and 1,3,7,and 14 days after tail suspension.The study protocol was approved by the Animal Experiment Ethics Committee of the PLA Strategic Support Force Characteristic Medical Center.RESULTS AND CONCLUSION:Intraocular pressure in the control group increased after tail suspension,which was significantly higher at 14 days after tail suspension than the baseline(P<0.05)as well as significantly higher at 7 days than 23 days after tail suspension(P<0.05).In the drug group,there was no significant difference in the intraocular pressure before and after tail suspension(P>0.05).Compared with the control group,the intraocular pressure was significantly reduced in the drug group(P<0.05).Retinal thickness showed no significant changes in each group before and after tail suspension(P>0.05),and there was also no significant difference between the two groups(P>0.05).In the control group,choroidal thickness was increased

关 键 词：模拟微重力环境 醋甲唑胺 眼压 视网膜厚度 脉络膜厚度 

分 类 号：R446[医药卫生—诊断学] R496[医药卫生—临床医学]