天津地区部分种公猪精液携带细菌性病原的检测与分析  

Detection and Analysis of Bacterial Pathogens in Boars Semen in Tianjin Area

作　　者：付永利 唐佩娟 崔捷 李文忠 杨婧 孙英峰 FU Yongli;TANG Peijuan;CUI Jie;LI Wenzhong;YANG Jing;SUN Yingfeng(Department of Animal Husbandry and Dairy Technology Promotion Service,Tianjin Agricultural Development Service Center,Tianjin 300061,China;College of Animal Science and Animal Medicine,Tianjin Agricultural University,Tianjin 300392,China)

机构地区：[1]天津市农业发展服务中心畜牧业及乳品技术推广服务部,天津300061 [2]天津农学院动物科学与动物医学学院,天津300384

出　　处：《天津农业科学》2021年第11期36-39,共4页Tianjin Agricultural Sciences

基　　金：天津市生猪产业技术体系创新团队项目——精细化养殖岗位(ITTPRS2021008)。

摘　　要：为了解天津地区种公猪精液中细菌的污染情况及耐药特征,具有针对性地添加抗菌药物,提高常温保存猪精液品质。本研究对2021年天津地区部分种猪场送检的494份精液样品进行了细菌分离,16S rDNA鉴定及耐药性分析研究。结果表明:天津地区公猪精液细菌携带比例高达38.87%(192/494),其中变形杆菌属(Proteus)占26.53%(52/192),葡萄球菌属(Staphylococcus)占23.96%(46/192),棒状杆菌属(Corynebacterium)占22.92%(44/192),肠球菌属(Enterococcus)占11.46%(22/192),大肠埃希氏菌属(Escherichia)占11.46%(22/192),未鉴定3.13%(6/192);耐药性分析显示5种细菌对四环素、林可霉素等已产生高度耐药,仅对喹诺酮类抗生素敏感,这为猪精液抗生素的使用提供参考。In order to understand the contamination and drug resistance of bacteria in boar semen in Tianjin area,the experiment collected 494 semen samples from breeding pig farms in Tianjin area in 2021 for bacterial isolation,identified the isolated bacteria by 16S rDNA,and then analyzed the drug resistance of the identified bacteria.The results showed that the rate of bacterial contamination was as high as 38.87%(192/494),of which Proteus was 26.53%(52/192),Staphylococcus was 23.96%(46/192),Corynebacterium was 22.92%(44/192),Enterococcus was 11.46%(22/192),Escherichia was 11.46%(22/192),and unidentified was 3.13%(6/192).Drug resistance analysis showed that these five bacteria species had high resistance to tetracycline and lincomycin,and were only sensitive to quinolones,which could use for semen preservation.

关 键 词：公猪精液 细菌鉴定 耐药分析 

分 类 号：S859.84[农业科学—临床兽医学]