长丰地区南瓜白粉病病原菌生理小种鉴定  

Identification of physiological race of pumpkin powdery mildew in Changfeng region

作　　者：夏伟伟 邓竹根 戴祖云 韦小飞 李曼[2] XIAWeiwei;DNEG Zhugen;DAI Zuyun;WEI Xiaofei;LI Man(Anhui Jianghuai Hortriculture Seeds Co.,Ltd.,Hefei 230031,Anhui,China;Anhui Agricultural University,Hefei 230036,Anhui,China)

机构地区：[1]安徽江淮园艺种业股份有限公司,合肥230031 [2]安徽农业大学,合肥230061

出　　处：《中国瓜菜》2021年第11期24-29,共6页China Cucurbits And Vegetables

基　　金：安徽省科技重大专项(202103a06020014);2020年安徽省领军人才团队项目。

摘　　要：为明确安徽省合肥市长丰地区南瓜白粉病病原菌种类及其生理小种,于2020年春季在长丰县吴山镇、水湖镇、岗集镇、造甲乡等地采集16份白粉病病原菌。通过观察病原菌的形态,并以长丰县吴山地区的白粉病菌总DNA为模板扩增其rDNA内转录间隔区(ITS)序列,经过测序比对,推断其为单囊壳白粉菌(Podosphaera xanthii)。最后采用国际公认的13个甜瓜白粉病生理小种鉴别寄主进行接种,根据抗感反应,确定长丰地区南瓜白粉病病原菌生理小种为Podosphaera xanthii race 1。初步明确了2020年春季合肥长丰地区南瓜白粉病病原菌的生理小种,为下一步该地区南瓜抗白粉病新品种选育奠定了基础。In order to verify the species and physiological races of pumpkin powdery mildew in Changfeng region,Hefei City,Anhui Province.In the spring of 2020,a total of 16 powdery mildew pathogens were collected in Wushan,Shuihu,Gangji,and Zaojia in Changfeng region.Through morphological observation,and using powdery mildew DNA from Wushan area of Changfeng region as a total DNA template to amplify its rDNA internal transcribed spacer(ITS)sequence.After sequencing and comparison,it was inferred to confirm that it was Podosphaera xanthii.Then the 13 internationally recognized race differenciation host were used to identify the races.Based on the resistance to susceptibility,Podosphaera xanthii physiology race 1 was determined as the pathogenic physiology race of pumpkin powdery mildew in Changfeng region on the spring of 2020.In this study,we had identified the physiological races of powdery mildew pathogen of pumpkin in Changfeng area of Hefei in the spring of 2020,which laid a foundation for breeding new varieties of pumpkin resistant to powdery mildew in this area.

关 键 词：南瓜 白粉病菌 生理小种 鉴定 

分 类 号：S642.1[农业科学—蔬菜学]